Division of Environmental Symbiosis Studies, Graduate School of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
Toxicol Lett. 2012 Dec 17;215(3):208-13. doi: 10.1016/j.toxlet.2012.10.012. Epub 2012 Oct 22.
It was recently reported that nanomolar concentrations of triclocarban, an antimicrobial agent, were detected in human blood after the use of soap containing triclocarban. Due to the widespread use of triclocarban in adult and infant personal care products, the report prompted us to study its cytotoxicity. The cytotoxicity of triclocarban was examined in rat thymocytes by using a cytometric technique with propidium iodide for examining cell lethality, FluoZin-3-AM for monitoring the intracellular Zn(2+) level, and 5-chloromethylfluorescencein diacetate for estimating the cellular content of non-protein thiol. The incubation with triclocarban at nanomolar concentrations (50-500nM) for 1h did not affect cell lethality but significantly elevated the intracellular Zn(2+) level. The elevation of the intracellular Zn(2+) level by triclocarban was not significantly dependent on external Zn(2+) level. There was a negative correlation (r=-0.9225) between the effect on the intracellular Zn(2+) level and that on the cellular content of non-protein thiol. These results suggest that nanomolar concentrations of triclocarban decrease the cellular content of non-protein thiol, leading to intracellular Zn(2+) release. Since zinc plays physiological roles in mammalian cells, the percutaneous absorption of triclocarban from soap may, therefore, affect some cellular functions.
最近有报道称，在使用含有三氯生的肥皂后，人血中检测到了纳米摩尔浓度的三氯生，一种抗菌剂。由于三氯生广泛用于成人和婴儿的个人护理产品，这一报道促使我们研究其细胞毒性。我们使用碘化丙啶检测细胞致死率、FluoZin-3-AM 监测细胞内 Zn(2+)水平以及 5-氯甲基荧光素二乙酸酯估计细胞内非蛋白巯基含量的细胞计量技术，研究了三氯生对大鼠胸腺细胞的细胞毒性。在孵育 1 小时的纳米摩尔浓度（50-500nM）下，三氯生不会影响细胞致死率，但显著提高了细胞内 Zn(2+)水平。三氯生引起的细胞内 Zn(2+)水平升高并不显著依赖于外部 Zn(2+)水平。细胞内 Zn(2+)水平的变化与非蛋白巯基含量的变化呈负相关（r=-0.9225）。这些结果表明，纳米摩尔浓度的三氯生降低了非蛋白巯基的细胞含量，导致细胞内 Zn(2+)释放。由于锌在哺乳动物细胞中发挥生理作用，因此肥皂中三氯生的经皮吸收可能会影响某些细胞功能。
