School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, United Kingdom.
Plasmid. 2013 Jan;69(1):96-103. doi: 10.1016/j.plasmid.2012.10.001. Epub 2012 Oct 23.
To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxycycline-induced transient overexpression was observed using vectors constructed with a combination of Tetracycline Responsive Element (TRE) and reverse tetracycline-controlled TransActivator advanced (rtTAadv), transfected in white (3T3-L1) and brown (HIB-1B) preadipocytes cell lines. The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes.
为了提供一个研究调节脂肪细胞分化的工具，构建了受脂肪特异性启动子控制的四环素诱导（Tet on）慢病毒表达载体。在没有强力霉素的情况下，观察到最低的基础表达，并且在使用包含四环素反应元件（TRE）和反式四环素控制转录激活剂高级（rtTAadv）的组合构建的载体中观察到最有效的剂量依赖性、强力霉素诱导的瞬时过表达，这些载体转染了白色（3T3-L1）和棕色（HIB-1B）前体脂肪细胞系。结果表明，在 HIB-1B 前体脂肪细胞中，使用 pLenti TRE / rtTA adv 可以实现截短的 aP2 启动子控制下的强力霉素诱导性脂肪生成表达。
