Biochefa Pharmaceutical Research and Production Plant, Sosnowiec, Poland.
Biol Trace Elem Res. 2012 Dec;150(1-3):509-12. doi: 10.1007/s12011-012-9524-4. Epub 2012 Oct 26.
Together with vitamin C, zinc, selenium, manganese, and magnesium play a vital role in the preservation of organs scheduled for transplantation. In the present study, it is shown that addition of 1 mg/l of these elements influences the stability of 0.3 mM ascorbic acid solutions. The solution's stability was estimated using an accelerated stability test. The concentration of vitamin C was measured using a validated spectrophotometric method, which uses the reduction of 2,6-dichlorophenoloindophenol by ascorbic acid. Elevated temperatures, the factor accelerating substances' decomposition reaction rate, were used in the tests. The research was conducted at two temperatures at intervals of 10 °C: 80 ± 0.1 and 90 ± 0.1 °C. It was stated that the studied substances' decomposition occurred in accordance with the equation for first-order reactions. The function of the logarithmic concentration (log%C) over time was revealed to be rectilinear. This dependence was used to determine the kinetics of decomposition reaction rate parameters. The stabilization of vitamin C solutions was measured as the time in which 10 % of the substance decomposed at 20 and 0 °C. Addition of Se(IV) or Mg(II) ions significantly increase the stability of ascorbic acid solution (∼34 and ∼16 %, respectively), but Zn(II) causes a significant decrease in stability by ∼23 %. Addition of Mn(II) has no significant influence on vitamin C stability.
与维生素 C 一起，锌、硒、锰和镁在维持预定移植器官的功能方面发挥着重要作用。在本研究中，表明添加 1mg/L 的这些元素会影响 0.3mM 抗坏血酸溶液的稳定性。使用加速稳定性测试来评估溶液的稳定性。使用经过验证的分光光度法测量维生素 C 的浓度，该方法利用抗坏血酸还原 2,6-二氯苯酚靛酚。在测试中使用升高的温度，即加速物质分解反应速率的因素。研究在两个间隔为 10°C 的温度下进行：80±0.1 和 90±0.1°C。研究表明，研究物质的分解符合一级反应方程。发现对数浓度（log%C）随时间的函数呈线性关系。该依赖性用于确定分解反应速率参数的动力学。维生素 C 溶液的稳定性通过在 20°C 和 0°C 下分解 10%物质所需的时间来测量。添加 Se(IV) 或 Mg(II)离子会显著增加抗坏血酸溶液的稳定性（分别约为 34%和 16%），但 Zn(II) 会使稳定性显著降低约 23%。添加 Mn(II) 对维生素 C 的稳定性没有显著影响。
