Ylönen J, Nuutinen J, Rautiainen M, Ruoppi P, Mäntyjärvi R, Virtanen T
Department of Clinical Microbiology, University of Kuopio, Finland.
Allergy. 1990 Jan;45(1):30-9. doi: 10.1111/j.1398-9995.1990.tb01081.x.
Bovine epithelial and urine antigen extracts were compared using enzyme-linked immunosorbent (ELISA) inhibition assay and sodium-dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with immunoblotting. According to ELISA inhibition results, the two extracts share about 2% of their antigenic components. Urine-derived antigens seem to be antigenically closer to serum proteins than epithelial antigens, as determined by rabbit immune sera. The IgG responses of allergic farmers against epithelial antigens were directed mainly against a protein of 20 kD, while non-allergic farmers had only very weak reactions. Generally, the IgG response against urinary antigens seemed to be more heterogeneous than against epithelial antigens. Almost all cow-allergic farmers reacted with a band at 20 kD in IgE immunoblotting against urinary and epithelial antigen while all non-allergic farmers showed negative results.
使用酶联免疫吸附（ELISA）抑制试验以及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）结合免疫印迹法，对牛上皮抗原提取物和尿液抗原提取物进行了比较。根据ELISA抑制结果，两种提取物约有2%的抗原成分相同。通过兔免疫血清测定，尿液来源的抗原在抗原性上似乎比上皮抗原更接近血清蛋白。过敏农民针对上皮抗原的IgG反应主要针对一种20kD的蛋白质，而非过敏农民的反应则非常微弱。一般来说，针对尿液抗原的IgG反应似乎比针对上皮抗原的反应更加多样化。几乎所有对牛过敏的农民在针对尿液和上皮抗原的IgE免疫印迹中，都与一条20kD的条带发生反应，而所有非过敏农民均呈阴性结果。
