Department of microbiology, St. Francis P.G. College for Women, Begumpet, Hyderabad, 500 016 India.
Indian J Microbiol. 2007 Dec;47(4):358-63. doi: 10.1007/s12088-007-0064-0. Epub 2008 Jan 11.
A marked reduction in uptake of α-santonin, accompanied by loss of ability of cells to transform the substrate, is observed on shocking Sphingomonas paucimobilis strain S ATCC 43388 cells by freeze - thaw method. The shock fluid shows a 26% quench in fluorescence at 350nm on incubation with the substrate. Addition of shock fluid to the freeze thawed cells restores both uptake as well as transformation of α-santonin to near normal.
用冻融法冲击鞘氨醇单胞菌 S 株（Sphingomonas paucimobilis strain S）ATCC 43388 细胞时，可观察到 α-当归酰欧前胡素的摄取明显减少，同时细胞失去转化底物的能力。在与底物孵育时，冲击液在 350nm 处的荧光猝灭率为 26%。将冲击液添加到冻融细胞中，可使 α-当归酰欧前胡素的摄取和转化恢复到接近正常水平。
