Vivekananda Institute of Hill Agriculture, Indian Council of Agricultural Research, Almora, 263 601 India.
Indian J Microbiol. 2010 Mar;50(1):50-6. doi: 10.1007/s12088-009-0024-y. Epub 2009 Apr 22.
Exiguobacterium acetylicum strain 1P (MTCC 8707) is a gram-positive, rod-shaped, yellow pigmented bacterium isolated from soil on nutrient agar plates at 4°C. The identity of the bacterium was arrived on the basis of the biochemical characterization, BIOLOG sugar utilization pattern and sequencing of the 16S rRNA gene. It grew at temperatures ranging from 4 to 42°C, with temperature optima at 30°C. It expressed multiple plant growth promotion attributes such as phosphate solubilization, indole acetic acid (IAA), siderophore and hydrogen cyanide (HCN) production, differentially at suboptimal growth temperatures (15 and 4°C). At 15°C it solubilized phosphate (21.1 μg of P ml(-1) day(-1)), and produced IAA (14.9 μg ml(-1) day(-1)) in tryptophan amended media. Qualitative detection of siderophore production and HCN were possible at 15°C. At 4°C it retained all the plant growth promotion attributes. Seed bacterization with the isolate, positively influenced the growth and nutrient uptake parameters of wheat seedlings in glass house studies at suboptimal cold growing temperatures.
极端小球菌 1P（MTCC 8707）菌株是一株革兰氏阳性、杆状、黄色素产生菌，从 4°C 的营养琼脂平板上的土壤中分离得到。该细菌的鉴定是基于生化特征、BIOLOG 糖利用模式和 16S rRNA 基因测序。它能在 4 到 42°C 的温度范围内生长，最适温度为 30°C。它表现出多种植物生长促进特性，如溶磷、吲哚乙酸（IAA）、铁载体和氢氰酸（HCN）的产生，在亚最适生长温度（15°C 和 4°C）下差异表达。在 15°C 时，它能溶解磷（21.1 μg P ml(-1) day(-1)），并在色氨酸添加的培养基中产生 IAA（14.9 μg ml(-1) day(-1)）。在 15°C 时可以定性检测到铁载体的产生和 HCN。在 4°C 时，它保留了所有的植物生长促进特性。在温室研究中，用该分离物对小麦种子进行细菌接种，对小麦幼苗在亚最适低温生长条件下的生长和养分吸收参数产生了积极影响。
