耐盐宏基因组克隆的序列分析。
Sequence analysis of a salt tolerant metagenomic clone.
机构信息
Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR), Mall Road, Delhi, 110 007 India ; Fermentation and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, 147 002 India.
出版信息
Indian J Microbiol. 2010 Jun;50(2):212-5. doi: 10.1007/s12088-010-0041-x. Epub 2010 Sep 16.
Abstract
Metagenome represent an unlimited resource for discovery of novel genes. Here we report, sequence analysis of a salt tolerant metagenomic clone (6B4) from a pond water metagenomic library. Clone 6B4 had an insert of 2254 bp with G+C composition of 64.06%. DNA sequence from 6B4 showed homology to DNA sequences from pro-teobacteria indicating origin of 6B4 metagenomic insert from a yet uncharacterized proteobacteria. Two encoded proteins from clone 6B4 showed match with ATP-depen-dent Clp protease adaptor protein (ClpS) and phasin, while two truncated encoded proteins showed match with poly-3-hydroxybutyrate synthase and permease. Clp complex is known to play a role in stress tolerance. Expression of ClpS from metagenomic clone is proposed to be responsible for salt tolerance of the metagenomic clone 6B4.
摘要
宏基因组代表了发现新基因的无限资源。在这里，我们报告了从池塘水宏基因组文库中分离的耐盐宏基因组克隆（6B4）的序列分析结果。克隆 6B4 的插入片段长 2254bp，G+C 含量为 64.06%。6B4 的 DNA 序列与来自变形菌门的 DNA 序列具有同源性，表明 6B4 宏基因组插入物来源于尚未鉴定的变形菌门。来自克隆 6B4 的两个编码蛋白与 ATP 依赖的 Clp 蛋白酶接头蛋白（ClpS）和 phasin 具有匹配性，而两个截断的编码蛋白与多聚 3-羟基丁酸合酶和透酶具有匹配性。Clp 复合物已知在应激耐受中发挥作用。推测宏基因组克隆中的 ClpS 表达负责宏基因组克隆 6B4 的耐盐性。
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