Department of Microbiology, Osmania University, Hyderabad, 500 007 India.
Indian J Microbiol. 2010 Sep;50(3):325-31. doi: 10.1007/s12088-010-0005-1. Epub 2010 Mar 5.
β-d-fructofuranosidase is one of the most important enzymes of the food industry especially due to its application in preparation of soft centered candies, confectioneries and to produce fructose syrups. Although several bacteria and filamentous fungi are reported for its production, yeasts are the most preferred source for this enzyme. In the present study mineral nutrients were screened for their effect on yeast growth and the enzyme production using Plackett-Burman design. Of the 11 nutrients screened, three variables (KH(2)PO(4), FeCl(3) and CoCl(2)) were significantly effecting yeast growth while six variables, i.e. KH(2)PO(4), CaCl(2), MnSO(4), Na(2)MoO(4), ZnSO(4) and CoCl(2) had significant effect on enzyme production.
β-呋喃果糖苷酶是食品工业中最重要的酶之一,特别是由于其在软心糖果、糖果和生产果葡糖浆中的应用。尽管有几种细菌和丝状真菌被报道用于生产这种酶,但酵母是这种酶的最优选来源。在本研究中,使用 Plackett-Burman 设计筛选了矿物质营养素对酵母生长和酶生产的影响。在所筛选的 11 种营养素中,有 3 种变量(KH(2)PO(4)、FeCl(3)和 CoCl(2))对酵母生长有显著影响,而 6 种变量,即 KH(2)PO(4)、CaCl(2)、MnSO(4)、Na(2)MoO(4)、ZnSO(4)和 CoCl(2)对酶生产有显著影响。