Zaĭtsev M G, Arliapov V A, Alferov V A, Reshetilov A N
Prikl Biokhim Mikrobiol. 2012 Sep-Oct;48(5):570-6.
Receptor elements for biosensors based on Hansenula polymorpha NCYC 495 In yeast cells for ethanol assay were developed using two ways of cell immobilization, i.e., physical adsorption on a glass fiber membrane and covalent binding on a modified nitrocellulose membrane. The linear diapason of ethanol assays for a biosensor based on yeast cells adsorbed on glass fiber was 0.05-1.18; for a biosensor based on yeasts immobilized on a nitrocellulose membrane, 0.2-1.53 mM. Receptor elements based on sorbed cells possessed 2.5 times higher long-term stability. The time response was 1.5 times less for cells immobilized using DEAE-dextran and benzochinone. The results of ethyl alcohol assays using biosensors based on cells immobilized via adsorption and covalent binding, as well as using the standard areometric method, had high correlation coefficients (0.998 and 0.997, respectively, for the two ways of immobilization). The results indicate the possibility to consider the described models of receptor elements for biosensors as prototypes for experimental samples for practical use.
基于多形汉逊酵母NCYC 495开发了用于酵母细胞乙醇检测的生物传感器受体元件，采用了两种细胞固定化方法，即在玻璃纤维膜上物理吸附和在改性硝酸纤维素膜上共价结合。基于吸附在玻璃纤维上的酵母细胞的生物传感器乙醇检测线性范围为0.05 - 1.18；基于固定在硝酸纤维素膜上的酵母的生物传感器，线性范围为0.2 - 1.53 mM。基于吸附细胞的受体元件具有高2.5倍的长期稳定性。使用DEAE - 葡聚糖和苯醌固定的细胞的时间响应少1.5倍。使用基于通过吸附和共价结合固定细胞的生物传感器以及使用标准等体积法进行乙醇检测的结果具有高相关系数（两种固定方法分别为0.998和0.997）。结果表明，可将所描述的生物传感器受体元件模型视为实际应用实验样品的原型。
