Korpan Ia I, Gonchar M V, Soldatkin A P, Starodub N F, Sandrovskiĭ A K, Sibirnyĭ A A, El'skaia A V
Ukr Biokhim Zh (1978). 1992 May-Jun;64(3):96-100.
Cellular sensors for methanol and ethanol determination were developed using immobilized mutant cells of methylotrophic yeasts Hansenula polymorpha and Pichia pinus (able to extrude protons in the presence of alcohol) and pH-sensitive field effect transistors (pH-SFETs). The intact cells of yeasts were immobilized in Ca-alginate gel to obtain a biomembrane. The minimal detectable response was obtained to approximately 0.5 mM of methanol and ethanol, a linear dependence of biosensor's response on the logarithmic alcohol concentration was observed in the range from 5 to 100 mM for both types of alcohol. The prospects for application of biosensors to determine alcohols in the analytical practice are discussed.
利用多形汉逊酵母和松毕赤酵母的固定化突变细胞（能够在酒精存在下排出质子）以及pH敏感场效应晶体管（pH-SFET）开发了用于测定甲醇和乙醇的细胞传感器。将酵母的完整细胞固定在海藻酸钙凝胶中以获得生物膜。对约0.5 mM的甲醇和乙醇获得了最小可检测响应，在两种类型的酒精浓度为5至100 mM的范围内观察到生物传感器响应与对数酒精浓度呈线性关系。讨论了生物传感器在分析实践中用于测定酒精的应用前景。
