Rozhkova I N, Brusentsev E Iu, Amstislavskiĭ S Ia
Ontogenez. 2012 Sep-Oct;43(5):309-19.
The structure and function of the mammalian oocyte and preimplantation embryo coverings are described in this review. The integrity of embryonic coverings is the main prerequisite for the success of such technology as preimplantation embryo freezing and, especially, for successful rederivation. On the other hand, results of in vitro fertilization and, sometimes, the results of embryo freezing are improved after perforation of the oocyte/embryonic coverings. Modern reproductive technologies focusing on oocyte/embryonic coverings, such as preimplantation embryo freezing/cryopreservation, in vitro fertilization, intracytoplasmic sperm injection, assisted hatching, immunocontraception, and rederivation, are reviewed. Application of these technologies to different mammalian species is discussed with a special emphasis on the oocytes/preimplantation embryos coverings.
本综述描述了哺乳动物卵母细胞和植入前胚胎覆盖物的结构与功能。胚胎覆盖物的完整性是诸如植入前胚胎冷冻等技术成功的主要前提，尤其是成功进行重新克隆的前提。另一方面，在卵母细胞/胚胎覆盖物穿孔后，体外受精的结果，有时还有胚胎冷冻的结果会得到改善。本文综述了聚焦于卵母细胞/胚胎覆盖物的现代生殖技术，如植入前胚胎冷冻/低温保存、体外受精、胞浆内单精子注射、辅助孵化、免疫避孕和重新克隆。讨论了这些技术在不同哺乳动物物种中的应用，并特别强调了卵母细胞/植入前胚胎覆盖物。
