[克氏锥虫重组SAPA蛋白在自然感染犬中的免疫酶学评估]

[Immunoenzymatic evaluation of the recombinant SAPA protein of Trypanosoma cruzi in naturally infected dogs].

作者信息

Cimino Rubén O, Diosque Patricio, López Quiroga Inés R, Gil José F, Nasser Julio R

机构信息

Cátedra de Química Biológica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Argentina.

出版信息

Rev Argent Microbiol. 2012 Jul-Sep;44(3):177-81.

PMID:23102466

Abstract

We evaluated the recombinant antigen SAPA (Shed Acute Phase Antigen) for the detection of Trypanosoma cruzi antibodies in sera from naturally infected dogs. The technique used was ELISA and the antigens were a homogenate of parasite T. cruzi (ELISA-H) and the recombinant SAPA (ELISA-SAPA). We analyzed 93 sera from dogs by ELISA-H and ELISA-SAPA, which were grouped as follows: G1: 11 negative control sera from the city of Salta, G2: 11 positive control sera from dogs naturally infected with T. cruzi and G3: 71 samples of dogs belonging to a Chagas disease-endemic area. The sensitivity and specificity of ELISA-SAPA were 100 %. The kappa index between ELISA-H and ELISA-SAPA was 0,85. These results confirm the use of SAPA antigen in the diagnosis of infection with T. cruzi in dogs.

摘要

我们评估了重组抗原SAPA（急性期脱落抗原）用于检测自然感染犬血清中克氏锥虫抗体的情况。所采用的技术为酶联免疫吸附测定（ELISA），抗原为克氏锥虫寄生虫匀浆（ELISA - H）和重组SAPA（ELISA - SAPA）。我们通过ELISA - H和ELISA - SAPA对93份犬血清进行了分析，这些血清分为以下几组：G1：来自萨尔塔市的11份阴性对照血清；G2：来自自然感染克氏锥虫犬的11份阳性对照血清；G3：来自恰加斯病流行区的71份犬样本。ELISA - SAPA的敏感性和特异性均为100%。ELISA - H和ELISA - SAPA之间的kappa指数为0.85。这些结果证实了SAPA抗原可用于犬克氏锥虫感染的诊断。