Foerster Claudia, Vidal Lorena, Troncoso Miriam, Figueroa Guillermo
Laboratorio de Microbiología y Probióticos, INTA, Universidad de Chile, Santiago, Chile.
Rev Argent Microbiol. 2012 Jul-Sep;44(3):195-200.
The aims of this study were to determine the occurrence of Listeria monocytogenes in cattle feces and ground beef, to characterize these strains by pulsed-field gel electrophoresis and to compare them to three listeria strains found in humans. Cattle from different origins (n = 250) and ground beef obtained from supermarkets (n = 40) were sampled. The results show low occurrence in cattle feces (0.4 %) but a higher presence in ground beef (37 %). An important part of the ground beef strains (80 %) had > 95 % similarity with a strain isolated from a human sporadic case and the ATCC 19115 used as control. The strain isolated from cattle feces had 93 % similarity to clone 009, previously associated with a listeriosis outbreak related to cheese. Cattle and ground beef can harbor virulent L. monocytogenes strains. Further studies in animals and animal products are needed to improve listeriosis control.
本研究的目的是确定牛粪便和绞碎牛肉中单核细胞增生李斯特菌的发生率，通过脉冲场凝胶电泳对这些菌株进行特征分析，并将它们与在人类中发现的三种李斯特菌菌株进行比较。对来自不同来源的牛（n = 250）和从超市获得的绞碎牛肉（n = 40）进行了采样。结果显示，牛粪便中的发生率较低（0.4%），但绞碎牛肉中的发生率较高（37%）。绞碎牛肉菌株的一个重要部分（80%）与从人类散发病例中分离出的菌株以及用作对照的ATCC 19115有> 95%的相似性。从牛粪便中分离出的菌株与克隆009有93%的相似性，克隆009先前与一起与奶酪相关的李斯特菌病暴发有关。牛和绞碎牛肉可能携带致病性单核细胞增生李斯特菌菌株。需要对动物和动物产品进行进一步研究，以改善李斯特菌病的控制。
