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来自人睾丸的环磷酸腺苷依赖性蛋白激酶I型α调节亚基的两种mRNA形式是由于使用了不同的聚腺苷酸化位点信号。

The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals.

作者信息

Sandberg M, Skålhegg B, Jahnsen T

机构信息

Laboratory for Gene Technology, University of Oslo, Norway.

出版信息

Biochem Biophys Res Commun. 1990 Feb 28;167(1):323-30. doi: 10.1016/0006-291x(90)91768-n.

DOI:10.1016/0006-291x(90)91768-n
PMID:2310396
Abstract

Using a cDNA probe for the type I alpha regulatory subunit, two mRNA species (1.5 and 3.0 kb in length) were detected in human testis. From a human testis cDNA-library a 3.0 kb clone, containing the entire reading frame of the protein, was isolated. Comparison of the nucleotide sequence of this clone to the sequence of a 1.5 kb cDNA clone earlier reported, showed that the longer clone was identical to the shorter but extended another 1.5 kb in the 3' end. Sequencing data together with Northern blot analysis indicated that the two mRNA species for human type I alpha regulatory subunit were generated from the same gene by the use of different polyadenylation site signals.

摘要

利用针对I型α调节亚基的cDNA探针，在人类睾丸中检测到两种mRNA（长度分别为1.5 kb和3.0 kb）。从人类睾丸cDNA文库中分离出一个3.0 kb的克隆，其包含该蛋白质的完整阅读框。将该克隆的核苷酸序列与先前报道的1.5 kb cDNA克隆的序列进行比较，结果显示较长的克隆与较短的克隆相同，但在3'端延伸了1.5 kb。测序数据以及Northern印迹分析表明，人类I型α调节亚基的两种mRNA是通过使用不同的聚腺苷酸化位点信号从同一基因产生的。

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