使用低压基因枪将生物弹丸DNA导入小鼠体内。

Biolistic DNA delivery to mice with the low pressure gene gun.

作者信息

Yen Meng-Chi, Lai Ming-Derg

机构信息

Department of Biochemistry and Molecular Biology, College of Medicine, Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan, ROC.

出版信息

Methods Mol Biol. 2013;940:169-74. doi: 10.1007/978-1-62703-110-3_14.

DOI:10.1007/978-1-62703-110-3_14

PMID:23104342

Abstract

Biolistic DNA delivery is an approach to deliver plasmid to culture cells, plants, or animals. Plasmid DNA is usually transferred through bombardment of DNA-coated particles by highly pressurized gas in various kinds of delivery vehicles. The low pressure gene gun can deliver plasmid at lower pressure. Here, we describe methods of biolistic DNA delivery to mice using the low pressure gene gun.

摘要

生物弹道DNA递送是一种将质粒递送至培养细胞、植物或动物的方法。质粒DNA通常通过在各种递送载体中用高压气体轰击包被有DNA的颗粒来转移。低压基因枪可以在较低压力下递送质粒。在此，我们描述了使用低压基因枪将生物弹道DNA递送至小鼠的方法。