用于检测班氏丝虫病的塞瓦菲拉克免疫测定法和快速免疫层析法丝虫病检测试验的评估
Evaluation of sevafilachek immunoassays and rapid ICT-filariasis test for detection of bancroftian filariasis.
作者信息
Alli R, Kulkarni S, Reddy M V, Harinath B C
机构信息
Department of Biochemistry & JB Tropical Disease Research Centre, MGIMS, 442102 Sevagram(Wardha), Maharashtra India.
出版信息
Indian J Clin Biochem. 2001 Jul;16(2):207-10. doi: 10.1007/BF02864863.
A comparative analysis was made on the utility of SEVAFILACHEK-stick based immunoassays and commercially available ICT-filariasis test to detect active infection in different groups of bancroftian filariasis. The SEVAFILACHEK immunoassays were found to be useful to detect filarial infection in microfilaraemia and in a significant number of clinical filarial cases with acute, chronic and occult clinical manifestations. In the clinical cases, microfilariae are not usually detected in peripheral circulation. Employing SEVAFILACHEK assays 6 and 5 of the 7 samples of patients with chronic filarial disease, and 6 and 5 of 6 microfilaraemic cases gave positivity for filarial IgG antibodies and antigen respectively. Four of the 6 occult filarial samples were positive for antibodies and antigen. Filarial antigen was detected by ICT-filariasis test in blood samples of all the 6 microfilariaemic cases, 1 chronic filarial and 2 occult filarial samples. The main advantage of ICT assay is its rapid format and convenience for field use.
对基于SEVAFILACHEK试纸条的免疫测定法和市售的ICT丝虫病检测法在检测不同组班氏丝虫病活动性感染中的效用进行了比较分析。结果发现，SEVAFILACHEK免疫测定法可用于检测微丝蚴血症患者以及大量具有急性、慢性和隐匿临床表现的临床丝虫病病例中的丝虫感染。在临床病例中，外周循环中通常检测不到微丝蚴。在慢性丝虫病患者的7份样本中，使用SEVAFILACHEK检测法，有6份和5份分别对丝虫IgG抗体和抗原呈阳性；在6例微丝蚴血症病例中，有6份和5份分别对丝虫IgG抗体和抗原呈阳性。6份隐匿性丝虫样本中有4份抗体和抗原呈阳性。在所有6例微丝蚴血症病例、1例慢性丝虫病病例和2例隐匿性丝虫病病例的血样中，通过ICT丝虫病检测法检测到了丝虫抗原。ICT检测法的主要优点是检测形式快速且便于现场使用。
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