Department of Pharmaceutical Engineering, Sangji University, Wonju-si, 220-702, Korea.
J Pharm Pharm Sci. 2012;15(4):519-27. doi: 10.18433/j39c76.
This study describes the development of a rapid and sensitive LC-ESI-MS assay for simultaneous enantioselective determination of levocetirizine and pseudoephedrine in dog plasma in the presence of dextrocetirizine.
Separations were achieved on an Ultron ES-OVM chiral column using the mobile phase consisting of 10 mM aqueous NH4OAc (pH 6.6) and acetonitrile (9:1 v/v).
The retention times of pseudoephedrine, dextrocetirizine, levocetirizine and diazepam (internal standard) were 5.2, 8.3, 9.6 and 11.6 min, respectively, and the total run time was less than 15 min. The assay was validated to demonstrate the linearity, accuracy and precision, recovery and stability. The calibration curves were linear over the concentration range from 1 - 200 ng/mL for levocetirizine and from 5 - 1000 ng/mL for pseudoephedrine.
The developed assay was successfully applied to a pharmacokinetic study after oral administration of the racemic cetirizine (0.5 mg/kg, or 0.25 mg/kg as levocetirizine) and pseudoephedrine (12 mg/kg) in the dog. This article is open to POST-PUBLICATION REVIEW. Registered readers (see "For Readers") may comment by clicking on ABSTRACT on the issue's contents page.
本研究描述了一种快速灵敏的 LC-ESI-MS 测定法，用于在存在右旋西替利嗪的情况下同时对犬血浆中的左西替利嗪和伪麻黄碱进行对映选择性测定。
在 Ultron ES-OVM 手性柱上，使用由 10 mM 水相 NH4OAc（pH 6.6）和乙腈（9:1 v/v）组成的流动相进行分离。
伪麻黄碱、右旋西替利嗪、左西替利嗪和地西泮（内标）的保留时间分别为 5.2、8.3、9.6 和 11.6 min，总运行时间小于 15 min。该测定法经过验证，证明了线性、准确性和精密度、回收率和稳定性。左西替利嗪的校准曲线在 1-200 ng/mL 浓度范围内呈线性，伪麻黄碱的校准曲线在 5-1000 ng/mL 浓度范围内呈线性。
该方法成功应用于犬口服西替利嗪（0.5 mg/kg，或左西替利嗪 0.25 mg/kg）和伪麻黄碱（12 mg/kg）后的药代动力学研究。本文接受 POST-PUBLICATION REVIEW。注册读者（见“读者须知”）可以在该问题的内容页面上点击 ABSTRACT 进行评论。
