Wei J Q, Wei J L, Zhou X T
Department of Internal Medicine, Affiliated Hospital of Shandong Medical University, Jinan, China.
Biomed Chromatogr. 1990 Jan;4(1):34-8. doi: 10.1002/bmc.1130040106.
An optimization strategy for an isocratic reversed phase high performance liquid chromatographic system (RP-HPLC) is described. Factorial design and a computer program are used to predict the retention time and resolution of fourteen steroids. An optimized rapid (less than 25 min) isocratic RP-HPLC system for the satisfactory separation of cortisone, cortisol, corticosterone, 11-deoxycortisol, 11-deoxycorticosterone, 17 alpha-hydroxyprogesterone, progesterone, androstenedione, testosterone, estrone, estradiol, estriol, prednisone acetate and dexamethasone acetate has been developed using this strategy through eight experiments.
