Department of Biology, University of Kentucky, 101 T. H. Morgan Building, Lexington, Kentucky 40506-0225 USA.
Am J Bot. 2012 Nov;99(11):e440-2. doi: 10.3732/ajb.1200187. Epub 2012 Oct 29.
Microsatellite markers were developed in Marchantia inflexa, a haploid liverwort with unisexual individuals, to identify clonal genotypes and measure population genetic variability.
Twelve polymorphic primer sets were developed from three enriched genomic libraries. Primers were fluorescently labeled, and alleles were identified by fragment analysis. These primers were tested in four natural populations and revealed a moderate level of genetic variation within four populations, as indicated by the number of alleles per locus (range = 1-5).
Development of polymorphic markers is crucial to the identification of individuals and will allow additional research into this species, particularly on its population genetics and metapopulation dynamics.
从单倍体地钱属植物（雌雄异株）中开发出微卫星标记，以识别克隆基因型并衡量种群遗传变异性。
从三个富集基因组文库中开发了 12 个多态性引物组。引物被荧光标记，通过片段分析来识别等位基因。这些引物在四个自然种群中进行了测试，结果表明四个种群内存在中等水平的遗传变异，每个位点的等位基因数量（范围为 1-5）。
多态性标记的开发对于个体识别至关重要，并将允许对该物种进行更多的研究，特别是在其种群遗传学和复合种群动态方面。
