Department of Biology, Ağrı İbrahim Çeçen University, Ağrı, Turkey.
Artif Cells Nanomed Biotechnol. 2013 Jun;41(3):213-21. doi: 10.3109/10731199.2012.716066. Epub 2012 Oct 30.
Poly(2-hydroxyethyl methacrylate)/3-(2-imidazoline-1-yl)propyl(triethoxysilane) (PHEMA/IMEO) nanoparticles were attached on surface plasmon resonance (SPR) sensor for the real-time detection of human immunoglobulin G (IgG) in human serum. The PHEMA/IMEO nanoparticles-attached SPR sensor was characterized by Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and contact angle measurements. IgG detection studies were performed using aqueous IgG solutions at different concentrations. In order to show the selectivity and specificity of the SPR sensor, competitive kinetic analyses were performed using IgG, albumin, hemoglobin in singular and competitive manner. Finally, IgG detection in human serum was carried out.
聚(2-羟乙基甲基丙烯酸酯)/3-(2-咪唑啉-1-基)丙基(三乙氧基硅烷)(PHEMA/IMEO)纳米粒子被附着在表面等离子体共振(SPR)传感器上，用于实时检测人血清中的人免疫球蛋白 G(IgG)。通过傅里叶变换红外光谱(FTIR)、原子力显微镜(AFM)和接触角测量对附着有 PHEMA/IMEO 纳米粒子的 SPR 传感器进行了表征。使用不同浓度的水溶液 IgG 溶液进行 IgG 检测研究。为了展示 SPR 传感器的选择性和特异性，使用 IgG、白蛋白、血红蛋白以单一组分和竞争方式进行了竞争性动力学分析。最后，在人血清中进行了 IgG 检测。
