Darden A G, Forbes R D, Darden P M, Guttmann R D
Transplantation Service, Royal Victoria Hospital, Montreal, Quebec, Canada.
Cell Immunol. 1990 Apr 1;126(2):322-30. doi: 10.1016/0008-8749(90)90324-k.
Interstitial dendritic cells (IDC) in normal hearts of inbred rat strains and congenic and congenic recombinant lines were identified and quantitated by immunohistologic methods, on the basis of cellular reactivity with MRC-OX6, a monoclonal antibody (MAb) directed against MHC class II determinants, and with W3/25, a MAb directed against a CD4 epitope. In all strains and lines examined, the W3/25+ IDC frequency was uniformly high with little interstrain variation. In contrast, all rat strains and lines examined showed either high or low OX6+ IDC frequency. Double staining by two color immunofluorescence indicated that strains with a low OX6+ IDC frequency were characterized by a high frequency of W3/25+ OX6- IDC and a low frequency of W3/25+ OX6+ IDC. In strains with high OX6+ IDC frequency, the majority of IDC coexpressed both markers. Comparative analysis of (i) MHC identical background disparate strains and lines, (ii) MHC disparate background identical strains and lines, and (iii) F2 segregation analysis of intercrosses and backcrosses derived from an original cross between high and low frequency OX6+ IDC strains, all indicated that OX6+ IDC frequency is dependent upon both MHC- and non-MHC-linked genetic factors. It is suggested that rat cardiac OX6+ IDC frequency is influenced by a minimum of two autosomal genes, one of which is MHC linked. The frequencies of both W3/25+ IDC frequency reached adult levels by 10 days of age; adult levels of OX6+ IDC were not attained until Day 21. It is postulated that in the rat heart, W3/25+ OX6- IDC are potential precursors of W3/25+ OX6+ IDC, and that the cellular frequency of coexpression in the adult is under genetic influence. Whether these genetic factors modify constitutive or physiologic levels of class II-inducing lymphokine activity, or influence cellular susceptibility of IDC to induced class II expression is unclear.
通过免疫组织学方法，基于细胞与MRC - OX6（一种针对MHC II类决定簇的单克隆抗体）和W3/25（一种针对CD4表位的单克隆抗体）的反应性，对近交系大鼠、同源系和同源重组系正常心脏中的间质树突状细胞（IDC）进行了鉴定和定量分析。在所有检测的品系和系中，W3/25 + IDC频率均较高，品系间差异较小。相比之下，所有检测的大鼠品系和系均表现出高或低的OX6 + IDC频率。双色免疫荧光双重染色表明，OX6 + IDC频率低的品系的特征是W3/25 + OX6 - IDC频率高，而W3/25 + OX6 + IDC频率低。在OX6 + IDC频率高的品系中，大多数IDC同时表达这两种标志物。对（i）MHC相同背景不同的品系和系、（ii）MHC不同背景相同的品系和系以及（iii）高频和低频OX6 + IDC品系之间原始杂交产生的杂交和回交的F2分离分析均表明，OX6 + IDC频率取决于MHC和非MHC连锁的遗传因素。提示大鼠心脏OX6 + IDC频率至少受两个常染色体基因影响，其中一个与MHC连锁。W3/25 + IDC频率在10日龄时达到成年水平；OX6 + IDC的成年水平直到第21天才达到。据推测，在大鼠心脏中，W3/25 + OX6 - IDC是W3/25 + OX6 + IDC的潜在前体，并且成年中共表达细胞频率受遗传影响。这些遗传因素是改变II类诱导淋巴因子活性的组成性或生理水平，还是影响IDC对诱导的II类表达的细胞敏感性尚不清楚。
