Departamento de Farmacología, Facultad de Medicina, UNAM, Av. Universidad 3000, UNAM, México D.F. 04510, México.
Ren Fail. 2013;35(1):132-7. doi: 10.3109/0886022X.2012.736071. Epub 2012 Nov 1.
The exposure to hexavalent chromium is often known to cause acute renal failure. It has been found that nonenzymatic antioxidants and the induction of heme oxygenase 1 have protective effects against nephrotoxicity induced by potassium dichromate in vivo. In this work, the effect of stannous chloride, an inducer of heme oxygenase 1, on potassium dichromate-induced toxicity in proximal tubular epithelial cells was studied. Hexavalent chromium levels, peroxynitrite content, reduced thiol content, heme oxygenase activity, reactive oxygen species production, and stannous chloride scavenging capacity were measured. It was found that stannous chloride protects proximal tubular epithelial cells from potassium dichromate-induced cell death. The decrease in extracellular and intracellular hexavalent chromium concentration, the induction of heme oxygenase 1, and the ability to scavenge reactive oxygen species and peroxynitrite are involved in the mechanism by which stannous chloride protects proximal tubular epithelial cells from potassium dichromate-induced toxicity.
六价铬的暴露通常会导致急性肾衰竭。已经发现非酶抗氧化剂和血红素加氧酶 1 的诱导对体内重铬酸钾引起的肾毒性具有保护作用。在这项工作中，研究了氯化亚锡（血红素加氧酶 1 的诱导剂）对近端肾小管上皮细胞中重铬酸钾诱导的毒性的影响。测量了六价铬水平、过氧亚硝酸盐含量、还原型巯基含量、血红素加氧酶活性、活性氧物质的产生以及氯化亚锡的清除能力。结果发现，氯化亚锡可保护近端肾小管上皮细胞免受重铬酸钾诱导的细胞死亡。氯化亚锡通过降低细胞外和细胞内六价铬浓度、诱导血红素加氧酶 1 以及清除活性氧物质和过氧亚硝酸盐的能力来保护近端肾小管上皮细胞免受重铬酸钾诱导的毒性。
