[Using reporter gene to compare infection efficiency of HIV-1 pseudovirus to suspended and adherent cells].

OBJECTIVE

In order to compare the infection of HIV-1 pseudovirus to suspended and adherent cells, Tzmbl cells containing beta-gal (beta-galactosidase) reporter gene were used here to do the analysis. pseudoviruses were generated by co-transfection of 293T cells with the plasmid pNL43R-E- and HIV envelope expressing plasmid. Supernatant of co-transfected 293T cells was collected and used to infect Tzmbl cells with or without trypsin treatment. Forty-eight hours after infection, beta-gal positive Tzmbl cells and virus infection were determined using X-gal staining and beta-glo (beta-galactosidase) assay.

RESULTS

The efficiency of HIV pseudoviruses infection of suspended Tzmbl cell was higher than that of adherent cells and the increase of infection correlated with the pseudoviral subtype.

CONCLUSION

This study may provide a useful method for HIV biological study and neutralization assays using a single-round replicative pseudovirus in the future.