Biological Sciences Division, Fundamental Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington 99352, United States.
J Am Chem Soc. 2012 Nov 14;134(45):18530-3. doi: 10.1021/ja308503x. Epub 2012 Nov 5.
Photoswitchable fluorescent probes capable of the targeted labeling of tagged proteins are of significant interest due to their ability to enable in situ imaging of protein complexes within native biomolecular assemblies. Here we describe the synthesis of a fluorescent probe (AsCy3Cy5) and demonstrate the targeted labeling and super-resolution imaging of a tagged protein within a supramolecular protein complex.
由于能够在天然生物分子组装体内部对蛋白质复合物进行原位成像，因此对能够对标记蛋白进行靶向标记的光可切换荧光探针具有重要的研究意义。在这里，我们描述了一种荧光探针（AsCy3Cy5）的合成，并展示了该探针对超分子蛋白质复合物内标记蛋白的靶向标记和超分辨成像。
