Department of Plastic and Reconstruction Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing 101199, PR China.
Oncol Rep. 2013 Jan;29(1):34-8. doi: 10.3892/or.2012.2089. Epub 2012 Oct 19.
The interleukin-6 (IL-6) pathway is one of the mechanisms that link inflammation and angiogenesis with malignancy. Since nuclear factor-κB (NF-κB) is a potential sign for inflammation, NF-κB has been associated with the progression of disease in various types of cancer. In the present study, we investigated the effect of NF-κB on the IL-6 pathway in gastric carcinoma and their correlation with disease status and prognosis. The mRNA and protein levels of NF-κB, IL-6 and vascular endothelial growth factor (VEGF) were detected by western blotting and reverse transcription (RT) quantitative PCR (RT-qPCR). Using immunohistochemistry, we examined the expression of these proteins in normal and human gastric cancer tissue samples. The concentrations of IL-6 and TNF-α in collected blood samples were measured according to the enzyme-linked immunosorbent assay (ELISA). IL-6 and TNF-α were found to be expressed at high levels in human gastric cancer samples. A positive correlation was found between the expression of IL-6 and NF-κB by immunohistochemical and further correlation analysis. IL-6, NF-κB and VEGF protein and mRNA levels increased significantly in gastric cancer tissue compared with those in adjacent normal mucosa tissue samples. In conclusion, our findings demonstrate that NF-κB, IL-6 and VEGF mRNA and protein levels increase significantly in gastric cancer tissues. In addition, the expression of NF-κB was positively correlated with the expression of IL-6 according to immunohistochemical and further correlation analysis, which suggests that the suppression of NF-κB or IL-6 may be a potential target for clinical therapy of gastric cancer in the future.
白细胞介素-6（IL-6）途径是将炎症和血管生成与恶性肿瘤联系起来的机制之一。由于核因子-κB（NF-κB）是炎症的潜在标志，因此 NF-κB 与各种类型癌症的疾病进展有关。在本研究中，我们研究了 NF-κB 对胃癌中 IL-6 途径的影响及其与疾病状态和预后的相关性。通过 Western blot 和逆转录（RT）定量 PCR（RT-qPCR）检测 NF-κB、IL-6 和血管内皮生长因子（VEGF）的 mRNA 和蛋白水平。使用免疫组织化学，我们检测了这些蛋白在正常和人胃癌组织样本中的表达。根据酶联免疫吸附测定（ELISA），测量了收集的血液样本中 IL-6 和 TNF-α 的浓度。发现人胃癌样本中 IL-6 和 TNF-α 的表达水平较高。通过免疫组织化学和进一步的相关性分析发现，IL-6 和 NF-κB 的表达呈正相关。与相邻正常黏膜组织样本相比，胃癌组织中 IL-6、NF-κB 和 VEGF 蛋白和 mRNA 水平显著增加。总之，我们的研究结果表明，胃癌组织中 NF-κB、IL-6 和 VEGF 的 mRNA 和蛋白水平显著增加。此外，根据免疫组织化学和进一步的相关性分析，NF-κB 的表达与 IL-6 的表达呈正相关，这表明抑制 NF-κB 或 IL-6 可能是未来胃癌临床治疗的潜在靶点。
