National Taiwan University Hospital and National Taiwan University College of Medicine, Department of Pediatrics, Taipei, 10002, Taiwan.
J Biomed Opt. 2012 Nov;17(11):116007. doi: 10.1117/1.JBO.17.11.116007.
Atopic dermatitis (AD) is an allergic inflammatory disease of skin. It remains unclear that CD207 of Langerhans cells (LCs) plays a central role in the development of allergic sensitization. There is little data on LCs within the microenviroment in vivo. We used a murine model of epicutaneous (EC) ovalbumin (OVA) sensitization inducing an inflammatory skin resembling AD to explore the role of CD207 in the pathogenesis of AD. Cr:forsterite laser-based multimodality nonlinear microscopy was applied for in situ imaging. Peritoneal injections of Alexa Fluor 647-rat anti-mouse CD207 into mice were performed to specifically trace the LCs. Peritoneal injections of OVA-Alexa Fluor 647 conjugate into mice were performed to specifically trace the OVA. We found that combining Alexa Fluor fluorescent probes with multimodality nonlinear microscopy permitted the unequivocal in situ imaging of CD207-expressing LCs. The relevant time-course, expressional, and functional studies reveal that CD207 of LCs plays an essential role during the induction of EC sensitization. We establish and validate that Cr:forsterite laser-based multimodality nonlinear microscopy is applicable for the specific detection of labeled mAb-bound LCs and labeled antigen. We suggest that CD207-expressing LCs initiate the allergic response through the CD207 mediated epicutaneous sensitization associated with the development of AD.
特应性皮炎（AD）是一种皮肤过敏炎症性疾病。朗格汉斯细胞（LCs）的 CD207 在过敏致敏的发展中起着核心作用，这一点尚不清楚。关于体内微环境中的 LCs，数据很少。我们使用经皮（EC）卵清蛋白（OVA）致敏诱导类似于 AD 的炎症性皮肤的小鼠模型，来探索 CD207 在 AD 发病机制中的作用。Cr：forsterite 基于激光的多模态非线性显微镜用于原位成像。通过向小鼠腹膜内注射 Alexa Fluor 647-抗鼠 CD207 来特异性追踪 LCs。通过向小鼠腹膜内注射 OVA-Alexa Fluor 647 缀合物来特异性追踪 OVA。我们发现，将 Alexa Fluor 荧光探针与多模态非线性显微镜相结合，可以明确原位成像表达 CD207 的 LCs。相关的时间过程、表达和功能研究表明，LCs 的 CD207 在 EC 致敏的诱导中起着至关重要的作用。我们建立并验证了 Cr：forsterite 基于激光的多模态非线性显微镜适用于标记 mAb 结合的 LCs 和标记抗原的特异性检测。我们认为，表达 CD207 的 LCs 通过与 AD 发展相关的 CD207 介导的经皮致敏引发过敏反应。
