Department of Food Animal Clinic, Chiang Mai University, Thailand.
J Microbiol Biotechnol. 2012 Nov;22(11):1467-70. doi: 10.4014/jmb.1112.12049.
We compared rapid fingerprinting using repetitive sequence-based PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n = 16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.
我们比较了使用基于重复序列的 PCR（rep-PCR）快速指纹分析对空肠弯曲杆菌分离株进行分型与广泛使用的多位点序列分型（MLST）。使用 MLST 和 rep-PCR 分析了来自肉鸡的代表性空肠弯曲杆菌分离株（n=16）。两种技术的区分能力均为 0.8917，鉴定出 9 个亚群。两种技术对所有 16 株分离株的克隆鉴定均相同。本研究中描述的 rep-PCR 可作为空肠弯曲杆菌分离株分型的快速且具有成本效益的替代方法，或作为大型流行病学研究中的有效筛选工具。
