Cecava M J, Merchen N R, Berger L L, Fahey G C
Dept. of Anim. Sci., University of Illinois, Urbana 61801.
J Anim Sci. 1990 Feb;68(2):467-77. doi: 10.2527/1990.682467x.
The intestinal supply of amino acids (AA) in sheep fed alkaline hydrogen peroxide-treated wheat straw (AHPWS)-based diets supplemented with soybean meal (SBM) or corn grain plus combinations of corn gluten meal (CGM) and blood meal (BM) was measured in a 5 X 5 latin square. Sheep (avg wt 45 kg) with ruminal, duodenal and ileal cannulas were fed diets containing 65% AHPWS supplemented with the following protein sources: soybean meal (SBM), corn gluten meal (CGM), blood meal (BM), 2/3 CGM:1/3 BM and 1/3 CGM:2/3 BM. Total nitrogen (N) flow at the duodenum was not affected (P greater than .05) by protein source. Flows of bacterial N and AA increased (P less than .05) and flows of nonbacterial N and AA decreased (P less than .05) when wethers were fed SBM vs corn plus other protein sources. When diets contained SBM, quantities of total AA at the duodenum were lower (P less than .05) and the profile of AA supplied to the intestine was altered substantially. Total flows of AA at the duodenum and total quantities of AA disappearing from the small intestine were similar (P greater than .05) for all diets containing BM and CGM, but flows and disappearance of valine, histidine, lysine and arginine increased linearly (P less than .05), whereas flows and disappearance of leucine, isoleucine and methionine decreased linearly (P less than .05) as BM replaced CGM in the diets. Results suggest that quantities of individual AA flowing to the duodenum and disappearing from the intestine of wethers fed AHPWS-based diets can be altered by source of dietary protein. Furthermore, feeding protein sources resistant to ruminal degradation in combination may improve the profile of AA supplied to the intestine.
在一个5×5拉丁方试验中,测定了以碱性过氧化氢处理麦秸(AHPWS)为基础日粮、补加豆粕(SBM)或玉米谷物以及玉米蛋白粉(CGM)与血粉(BM)组合的绵羊肠道氨基酸(AA)供应量。给装有瘤胃、十二指肠和回肠瘘管的绵羊(平均体重45千克)饲喂含65% AHPWS并补加以下蛋白质来源的日粮:豆粕(SBM)、玉米蛋白粉(CGM)、血粉(BM)、2/3 CGM:1/3 BM和1/3 CGM:2/3 BM。十二指肠总氮(N)流量不受蛋白质来源的影响(P>0.05)。与饲喂玉米加其他蛋白质来源相比,阉羊饲喂SBM时细菌N和AA流量增加(P<0.05),非细菌N和AA流量减少(P<0.05)。当日粮含有SBM时,十二指肠总AA量较低(P<0.05),供应至肠道的AA谱发生显著改变。对于所有含BM和CGM的日粮,十二指肠AA总流量和从小肠消失的AA总量相似(P>0.05),但随着日粮中BM替代CGM,缬氨酸、组氨酸、赖氨酸和精氨酸的流量和消失量呈线性增加(P<0.05),而亮氨酸、异亮氨酸和蛋氨酸的流量和消失量呈线性减少(P<0.05)。结果表明,饲喂以AHPWS为基础日粮的阉羊,流向十二指肠并从小肠消失的单个AA量可因日粮蛋白质来源而改变。此外,组合饲喂抗瘤胃降解的蛋白质来源可能会改善供应至肠道的AA谱。