Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, Nanjing 210095, People's Republic of China.
Bioresour Technol. 2013 Jan;127:337-42. doi: 10.1016/j.biortech.2012.09.116. Epub 2012 Oct 9.
A bacterial strain, Cupriavidus sp. DT-1, capable of degrading chlorpyrifos and 3,5,6-trichloro-2-pyridinol (TCP) and using these compounds as sole carbon source was isolated and characterized. Investigation of the degradation pathway showed that chlorpyrifos was first hydrolyzed to TCP, successively dechlorinated to 2-pyridinol, and then subjected to the cleavage of the pyridine ring and further degradation. The mpd gene, encoding the enzyme responsible for chlorpyrifos hydrolysis to TCP, was cloned and expressed in Escherichia coli BL21. Inoculation of chlorpyrifos-contaminated soil with strain DT-1 resulted in a degradation rate of chlorpyrifos and TCP of 100% and 94.3%, respectively as compared to a rate of 28.2% and 19.9% in uninoculated soil. This finding suggests that strain DT-1 has potential for use in bioremediation of chlorpyrifos-contaminated environments.
一株能够降解毒死蜱和 3,5,6-三氯-2-吡啶醇（TCP）并将这些化合物作为唯一碳源的细菌菌株 Cupriavidus sp. DT-1 被分离和鉴定。降解途径的研究表明，毒死蜱首先被水解为 TCP，然后依次脱氯生成 2-吡啶醇，接着进行吡啶环的断裂和进一步降解。mpd 基因，编码负责将毒死蜱水解为 TCP 的酶，被克隆并在大肠杆菌 BL21 中表达。与未接种土壤相比，用 DT-1 菌株接种污染土壤后，毒死蜱和 TCP 的降解率分别达到 100%和 94.3%，而未接种土壤的降解率分别为 28.2%和 19.9%。这一发现表明，DT-1 菌株具有在污染环境中进行生物修复的潜力。
Zotero 插件