澳大利亚和加拿大马铃薯卷叶黄化病毒分离株的核苷酸序列及其与两种欧洲分离株的关系。

Nucleotide sequences of an Australian and a Canadian isolate of potato leafroll luteovirus and their relationships with two European isolates.

作者信息

Keese P, Martin R R, Kawchuk L M, Waterhouse P M, Gerlach W L

机构信息

CSIRO Division of Plant Industry, Canberra ACT, Australia.

出版信息

J Gen Virol. 1990 Mar;71 ( Pt 3):719-24. doi: 10.1099/0022-1317-71-3-719.

DOI:10.1099/0022-1317-71-3-719

PMID:2313269

Abstract

The genomes of an Australian and a Canadian isolate of potato leafroll virus have been cloned and sequenced. The sequences of both isolates are similar (about 93%), but the Canadian isolate (PLRV-C) is more closely related (about 98% identity) to a Scottish (PLRV-S) and a Dutch isolate (PLRV-N) than to the Australian isolate (PLRV-A). The 5'-terminal 18 nucleotide residues of PLRV-C, PLRV-A, PLRV-N and beet western yellows virus have 17 residues in common. In contrast, PLRV-S shows no obvious similarity in this region. PLRV-A and PLRV-C genomic sequences have localized regions of marked diversity, in particular a 600 nucleotide residue sequence in the polymerase gene. These data provide a world-wide perspective on the molecular biology of PLRV strains and their comparison with other luteoviruses and related RNA plant viruses suggests that there are two major subgroups in the plant luteoviruses.

摘要

已对澳大利亚和加拿大分离出的马铃薯卷叶病毒基因组进行了克隆和测序。两种分离株的序列相似（约93%），但加拿大分离株（PLRV-C）与苏格兰分离株（PLRV-S）和荷兰分离株（PLRV-N）的亲缘关系更近（约98%的同一性），而与澳大利亚分离株（PLRV-A）的亲缘关系较远。PLRV-C、PLRV-A、PLRV-N和甜菜西方黄化病毒的5'端18个核苷酸残基有17个是相同的。相比之下，PLRV-S在该区域没有明显的相似性。PLRV-A和PLRV-C基因组序列有明显多样性的局部区域，特别是在聚合酶基因中有一个600个核苷酸残基的序列。这些数据为PLRV毒株的分子生物学提供了一个全球视角，并且将它们与其他黄症病毒及相关RNA植物病毒进行比较表明，植物黄症病毒存在两个主要亚组。