de Miranda J R, Stevens M, de Bruyne E, Smith H G, Bird C, Hull R
John Innes Centre, Colney, Norwich, U.K.
Arch Virol. 1995;140(12):2183-200. doi: 10.1007/BF01323239.
Three distinct sequence groups were found among partial nucleotide sequences of 38 isolates of beet western yellows virus (BWYV) and beet mild yellowing virus (BMYV) from Europe, Iran and the USA. The first group contains both sugar beet and oilseed rape specific isolates, and the differentiating characteristic linked to this host range specificity are 2 single base pair changes in a 1,200 nucleotide region of the genome. It is proposed that the European BWYV strains that can be transferred at low frequency between rape and sugar beet belong to this group. Also belonging to this group are the published BWYV sequences of Veidt et al. and of the California BWYV-ST9 isolate. The second group contains mostly rape-derived isolates which have an intergenic region highly distinct from that of group-1 isolates but similar polymerase and coat protein regions. It is proposed that the rape-specific BWYV isolates which cannot be transmitted to sugar beet belong to this group. The third group contains mostly beet-specific isolates from Southern Europe and Iran, and may be adapted to the Mediterranean climate and flora. It is distinct from groups 1 and 2 in all three genome regions investigated and its polymerase and intergenic regions are as much related to those of potato leafroll virus (PLRV) and curcurbit aphid borne yellows virus (CABYV) as they are to those of group-1 and group-2. On the basis of sequence similarities and established nomenclature it is proposed to use BWYV for groups 1 and 2 (BWYV-1 and BWYV-2 respectively) and to use BMYV for group-3 isolates, which are distinct enough from the other two groups to merit a separate nomenclature.
在来自欧洲、伊朗和美国的38株甜菜西方黄化病毒（BWYV）和甜菜轻度黄化病毒（BMYV）的部分核苷酸序列中，发现了三个不同的序列组。第一组包含甜菜和油菜特定的分离株，与这种宿主范围特异性相关的区别特征是基因组1200个核苷酸区域中的2个单碱基对变化。据推测，能够在油菜和甜菜之间低频率转移的欧洲BWYV毒株属于该组。Veidt等人公布的BWYV序列以及加利福尼亚BWYV-ST9分离株也属于该组。第二组主要包含来自油菜的分离株，其基因间隔区与第一组分离株的基因间隔区高度不同，但聚合酶和外壳蛋白区域相似。据推测，不能传播到甜菜的油菜特异性BWYV分离株属于该组。第三组主要包含来自南欧和伊朗的甜菜特异性分离株，可能适应地中海气候和植物群。在所研究的所有三个基因组区域中，它与第一组和第二组不同，其聚合酶和基因间隔区与马铃薯卷叶病毒（PLRV）和葫芦科蚜传黄化病毒（CABYV）的聚合酶和基因间隔区的相关性与它们与第一组和第二组的相关性一样高。基于序列相似性和既定的命名法，建议将第一组和第二组分别使用BWYV（分别为BWYV-1和BWYV-2），而第三组分离株使用BMYV，它们与其他两组明显不同，值得单独命名。
