Naz Ruphi, Anis M, Aref I M
Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202 002, India.
Acta Biol Hung. 2012 Dec;63(4):474-82. doi: 10.1556/ABiol.63.2012.4.6.
An efficient and reproducible protocol for in vitro multiplication of Bauhinia tomentosa L. was developed. Multiple shoots were regenerated from cotyledonary node and stem nodal segments excised from in vitro raised seedlings on Murashige and Skoog (MS) medium supplemented with different concentrations (0.1, 0.3, 0.5, 0.8 and 1.0 μM) of thidiazuron (TDZ). The maximum response (62.6%) was recorded on MS medium amended with 0.8 μM TDZ. A long exposure to TDZ for 8 weeks showed abnormalities such as fasciation and compact shoots formation. To avoid adverse effects of prolonged exposure to TDZ in long-term establishment, the culture were transferred to TDZ free MS medium for further multiplication and elongation. The highest number of shoots and shoot length were recorded at the end of fourth subculture passage. Ex vitro rooting was achieved when the basal cut end of regenerated shoots were dipped in 200 μM indole-3-butyric acid (IBA) for half an hour followed by their transplantation in plastic pots filled with sterile Soilrite™ where 60% plantlets grew well and all expressed normal development.
开发了一种高效且可重复的绒毛羊蹄甲体外增殖方案。从在添加不同浓度（0.1、0.3、0.5、0.8 和 1.0 μM）噻苯隆（TDZ）的 Murashige 和 Skoog（MS）培养基上的体外培养幼苗切下的子叶节和茎节段再生出多个芽。在用 0.8 μM TDZ 改良的 MS 培养基上记录到最大响应率（62.6%）。长时间暴露于 TDZ 8 周会出现诸如扁化和紧凑芽形成等异常情况。为避免长期培养中长时间暴露于 TDZ 的不利影响，将培养物转移到不含 TDZ 的 MS 培养基上进行进一步增殖和伸长。在第四次继代培养结束时记录到最高的芽数和芽长度。当将再生芽的基部切割端浸入 200 μM 吲哚 - 3 - 丁酸（IBA）中半小时，然后移植到装有无菌 Soilrite™ 的塑料盆中时，实现了离体生根，其中 60% 的植株生长良好且均表现出正常发育。
