Institute of Biotechnology, College of Engineering, Southern Taiwan University of Science and Technology, Tainan, Taiwan.
Chin Med. 2012 Nov 7;7(1):24. doi: 10.1186/1749-8546-7-24.
Xiao-Yao-San (XYS) is a Chinese medicinal formula for treating anxiety and depression. This study aims to evaluate the use of a room-temperature super-extraction system (RTSES) to extract the major active components of XYS and enhance their psycho-pharmacological effects.
The neuroprotective roles of XYS/RTSES against reserpine-derived neurotoxicity were evaluated using a glial cell injury system (in vitro) and a depression-like C57BL/6 J mouse model (in vivo). The anxiolytic-behavioural effects were measured by the elevated plus-maze (EPM) test and the antidepressant effects were evaluated by the forced swimming test (FST) and tail suspension test (TST). Glucose tolerance and insulin resistance were assayed by ELISA. The expression of 5-HT1A receptors in the prefrontal cortex was examined by western blotting.
XYS/RTSES (300 μg/mL) diminished reserpine-induced glial cell death more effectively than either XYS (300 μg/mL) or fluoxetine (30 μM) at 24 h (P = 0.0481 and P = 0.054, respectively). Oral administration of XYS/RTSES (500 mg/kg/day) for 4 consecutive weeks significantly elevated the ratios of entries (open arms/closed arms; P = 0.0177) and shuttle activity (P = 0.00149) on the EPM test, and reduced the immobility time by 90% on the TST (P = 0.00000538) and FST (P = 0.0000053839). XYS/RTSES also improved the regulation of blood glucose (P = 0.0305) and increased the insulin sensitivity (P = 0.0093). The Western blot results indicated that the activation of cerebral 5-HT1A receptors may be involved in the mechanisms of XYS/RTSES actions.
The RTSES could provide a novel method for extracting effective anxiolytic- and antidepressant-like substances. XYS/RTSES improved the regulation of blood glucose and increased the insulin sensitivity in reserpine-induced anxiety and depression. Neuroprotection of glial cells and activation of cerebral 5-HT1A receptors were also involved.
逍遥散（XYS）是一种治疗焦虑和抑郁的中药方剂。本研究旨在评估使用室温超萃取系统（RTSES）提取 XYS 的主要活性成分并增强其精神药理学作用。
采用体外胶质细胞损伤系统和抑郁样 C57BL/6 J 小鼠模型（体内）评价 XYS/RTSES 对利血平诱导的神经毒性的神经保护作用。采用高架十字迷宫（EPM）试验测定抗焦虑行为效应，采用强迫游泳试验（FST）和悬尾试验（TST）评价抗抑郁作用。通过 ELISA 测定葡萄糖耐量和胰岛素抵抗。采用 Western blot 检测前额叶皮质 5-HT1A 受体的表达。
XYS/RTSES（300μg/ml）在 24 小时内比 XYS（300μg/ml）或氟西汀（30μM）更有效地减少利血平诱导的神经胶质细胞死亡（P=0.0481 和 P=0.054）。连续 4 周口服 XYS/RTSES（500mg/kg/天）显著提高 EPM 试验中进入（开放臂/封闭臂）的比例（P=0.0177）和穿梭活性（P=0.00149），并使 TST（P=0.00000538）和 FST（P=0.0000053839）中的不动时间减少 90%。XYS/RTSES 还改善了血糖调节（P=0.0305），增加了胰岛素敏感性（P=0.0093）。Western blot 结果表明，脑 5-HT1A 受体的激活可能参与了 XYS/RTSES 作用的机制。
RTSES 可为提取有效的抗焦虑和抗抑郁样物质提供一种新方法。XYS/RTSES 改善了利血平诱导的焦虑和抑郁的血糖调节，增加了胰岛素敏感性。胶质细胞的神经保护和脑 5-HT1A 受体的激活也参与其中。
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