Institut des Sciences du Végétal, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette cedex, France.
Plant Cell. 2012 Nov;24(11):4498-510. doi: 10.1105/tpc.112.103747. Epub 2012 Nov 6.
During their symbiotic interaction with rhizobia, legume plants develop symbiosis-specific organs on their roots, called nodules, that house nitrogen-fixing bacteria. The molecular mechanisms governing the identity and maintenance of these organs are unknown. Using Medicago truncatula nodule root (noot) mutants and pea (Pisum sativum) cochleata (coch) mutants, which are characterized by the abnormal development of roots from the nodule, we identified the NOOT and COCH genes as being necessary for the robust maintenance of nodule identity throughout the nodule developmental program. NOOT and COCH are Arabidopsis thaliana BLADE-ON-PETIOLE orthologs, and we have shown that their functions in leaf and flower development are conserved in M. truncatula and pea. The identification of these two genes defines a clade in the BTB/POZ-ankyrin domain proteins that shares conserved functions in eudicot organ development and suggests that NOOT and COCH were recruited to repress root identity in the legume symbiotic organ.
在与根瘤菌共生的过程中，豆科植物在其根部形成共生特有的器官，称为根瘤，其中含有固氮细菌。控制这些器官的身份和维持的分子机制尚不清楚。我们使用苜蓿根瘤突变体（noot）和豌豆 cochleata（coch）突变体，这些突变体的特征是根瘤中的根异常发育，鉴定出 NOOT 和 COCH 基因是整个根瘤发育过程中维持根瘤身份的必需基因。NOOT 和 COCH 是拟南芥 BLADE-ON-PETIOLE 同源物，我们已经证明它们在叶和花发育中的功能在苜蓿和豌豆中是保守的。这两个基因的鉴定定义了 BTB/POZ-ankyrin 结构域蛋白中的一个分支，在真双子叶植物器官发育中具有保守的功能，并表明 NOOT 和 COCH 被招募来抑制豆科共生器官中的根身份。
