Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
Eur J Med Chem. 2012 Dec;58:308-16. doi: 10.1016/j.ejmech.2012.09.051. Epub 2012 Oct 18.
Metal-based cancer chemotherapeutic agents of the type [Cu(phen)TzCl(2)]H(2)O 1 and [Zn(phen)(Tz)Cl(2)·H(2)O] 2, where phen = 1,10-phenanthroline and Tz = 1,2,4-triazole have been synthesized and characterized by various spectroscopic and analytical techniques. The structure of complex 1 was also determined by X-ray crystallography. The in vitro DNA binding studies of complexes 1 and 2 with CT DNA were carried out by various biophysical and molecular docking techniques. Both the complexes cleave supercoiled pBR322 DNA via hydrolytic pathway, as validated by T4 DNA ligase assay. Furthermore, both complexes exhibited significant antimicrobial activity. The results revealed that complex 1 has better prospectus to act as cancer chemotherapeutic candidate which warrants further in vitro and in vivo anticancer investigations.
[Cu(phen)TzCl(2)]H(2)O1 和 [Zn(phen)(Tz)Cl(2)·H(2)O]2，其中 phen = 1,10-菲咯啉，Tz = 1,2,4-三唑。通过 X 射线晶体学确定了配合物 1 的结构。通过各种生物物理和分子对接技术研究了配合物 1 和 2 与 CT DNA 的体外 DNA 结合。T4 DNA 连接酶测定验证了两种配合物均通过水解途径切割超螺旋 pBR322 DNA。此外，两种配合物都表现出显著的抗菌活性。结果表明，配合物 1 具有更好的作为癌症化疗候选物的前景，值得进一步进行体外和体内抗癌研究。
