National Reference Centre for Legionella pneumophila, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
Euro Surveill. 2012 Oct 25;17(43):20302.
Sequence-based typing (SBT) is a discriminatory method widely used to genotype Legionella pneumophila strains. A total of 86 clinical L. pneumophila serogroup 1 (sg1) isolates, collected between January 2000 and December 2010 in the two Belgian National Reference Centres for Legionella pneumophila, were genotyped using the internationally standardised SBT protocol of the European Working Group for Legionella Infections (EWGLI). The isolates could be classified into 31 different sequence types (ST, index of diversity: 0.879). The obtained STs were submitted to the EWGLI SBT-database for L. pneumophila. In our study, ST47 (27.9%) and ST1 (19.8%) were the most frequently detected STs. The detected profiles were a combination of both frequently isolated and unique STs, and of both worldwide distributed and more local strains. Two STs, ST880 and ST881, were new to the EWGLI database. In conclusion, we characterised L. pneumophila sg1 isolates with the SBT method, and created a Belgian profile database that will be useful for future epidemiological studies.
序列分型（SBT）是一种广泛用于基因分型嗜肺军团菌菌株的鉴别方法。2000 年 1 月至 2010 年 12 月期间，在比利时的两个国家嗜肺军团菌参考中心收集了总共 86 株临床嗜肺军团菌血清型 1（sg1）分离株，使用国际标准化的欧洲军团菌感染工作组（EWGLI）SBT 方案对其进行了基因分型。这些分离株可分为 31 种不同的序列型（ST，多样性指数：0.879）。获得的 ST 被提交给 EWGLI SBT 嗜肺军团菌数据库。在我们的研究中，ST47（27.9%）和 ST1（19.8%）是最常检测到的 ST。检测到的谱是经常分离的和独特的 ST 的组合，也是分布在世界各地的和更局部的菌株的组合。两个 ST，ST880 和 ST881，是 EWGLI 数据库中的新菌株。总之，我们用 SBT 方法对嗜肺军团菌 sg1 分离株进行了特征描述，并创建了一个比利时的特征数据库，这将对未来的流行病学研究有用。
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