吸烟者的阴道细胞比不吸烟者更能抵抗人乳头瘤病毒感染。
Vaginal cells of smokers are more resistant to human papillomavirus infection than that of non-smokers.
机构信息
Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40202, USA.
出版信息
Exp Mol Pathol. 2012 Dec;93(3):422-7. doi: 10.1016/j.yexmp.2012.10.016. Epub 2012 Nov 5.
To evaluate effect of HPV and smoking on DNA double-strand breaks in vaginal samples, vaginal specimens collected from participants (n=76) were classified based on HPV and smoking status, and DNA double-strand breaks measured using comet assay. Mean tail length (31.2±18.7μm) and tail moment (2.4±2.8 arbitrary units) for HPV-positive patients were lower (p<0.001) compared with HPV-negative patients (61.7±22.6μm; 8.7±4.9AU). Never-smokers were found to have a higher level (p<0.001) of double-strand breaks (57.7±24.5μm, 7.5±5.5AU) compared with ever smokers (35.3±21.9μm; 3.4±3.7AU). Among HPV infected patients, never-smokers have more double-strand breaks compared to smokers (p<0.001) which correlated with age (p<0.001). Highly differentiated vaginal epithelium may be resistant to DNA damage associated with HPV infection and smoking, which may be attributed to adoptive survival mechanisms of vaginal epithelium.
为了评估 HPV 和吸烟对阴道样本中 DNA 双链断裂的影响，根据 HPV 和吸烟状况对 76 名参与者的阴道标本进行分类，并使用彗星试验测量 DNA 双链断裂。与 HPV 阴性患者（61.7±22.6μm；8.7±4.9AU）相比，HPV 阳性患者的平均尾部长度（31.2±18.7μm）和尾部矩（2.4±2.8 任意单位）较低（p<0.001）。从未吸烟者的双链断裂水平（57.7±24.5μm，7.5±5.5AU）高于吸烟者（35.3±21.9μm，3.4±3.7AU）（p<0.001）。在 HPV 感染患者中，从未吸烟者的双链断裂比吸烟者更多（p<0.001），这与年龄相关（p<0.001）。高度分化的阴道上皮可能对与 HPV 感染和吸烟相关的 DNA 损伤具有抗性，这可能归因于阴道上皮的适应性生存机制。
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