Centre for Plant Molecular Biology, Osmania University, Hyderabad 500007, India.
Gene. 2013 Jan 15;513(1):156-62. doi: 10.1016/j.gene.2012.10.040. Epub 2012 Nov 6.
Flax CYPome analysis resulted in the identification of 334 putative cytochrome P450 (CYP450) genes in the cultivated flax genome. Classification of flax CYP450 genes based on the sequence similarity with Arabidopsis orthologs and CYP450 nomenclature, revealed 10 clans representing 44 families and 98 subfamilies. CYP80, CYP83, CYP92, CYP702, CYP705, CYP708, CYP728, CYP729, CYP733 and CYP736 families are absent in the flax genome. The subfamily members exhibited conserved sequences, length of exons and phasing of introns. Similarity search of the genomic resources of wild flax species Linum bienne with CYP450 coding sequences of the cultivated flax, revealed the presence of 127 CYP450 gene orthologs, indicating amplification of novel CYP450 genes in the cultivated flax. Seven families CYP73, 74, 75, 76, 77, 84 and 709, coding for enzymes associated with phenylpropanoid/fatty acid metabolism, showed extensive gene amplification in the flax. About 59% of the flax CYP450 genes were present in the EST libraries.
亚麻 CYPome 分析鉴定出栽培亚麻基因组中 334 个假定细胞色素 P450（CYP450）基因。根据与拟南芥同源物和 CYP450 命名法的序列相似性对亚麻 CYP450 基因进行分类，揭示了代表 44 个家族和 98 个子家族的 10 个族。CYP80、CYP83、CYP92、CYP702、CYP705、CYP708、CYP728、CYP729、CYP733 和 CYP736 家族在亚麻基因组中缺失。亚家族成员表现出保守的序列、外显子长度和内含子相位。与栽培亚麻 CYP450 编码序列的野生亚麻物种 Linum bienne 的基因组资源进行相似性搜索，发现了 127 个 CYP450 基因直系同源物，表明在栽培亚麻中扩增了新的 CYP450 基因。与苯丙烷/脂肪酸代谢相关的酶编码的 7 个家族 CYP73、74、75、76、77、84 和 709，显示出在亚麻中广泛的基因扩增。约 59%的亚麻 CYP450 基因存在于 EST 文库中。
