硒对1,2 - 二甲基肼诱导的大鼠结肠癌发生过程中癌症标志物指标及超微结构变化的化学预防作用。

Chemopreventive effects of selenium on cancer marker indices and ultrastructural changes during 1,2 dimethylhydrazine-induced colon carcinogenesis in rats.

作者信息

Ghadi Fereshteh Ezzati, Malhotra Anshoo, Ghara Abdollah Ramzani, Dhawan D K

机构信息

Department of Biophysics, Panjab University, Chandigarh, 160 014, India.

出版信息

J Gastrointest Cancer. 2013 Mar;44(1):54-9. doi: 10.1007/s12029-012-9451-9.

DOI:10.1007/s12029-012-9451-9

PMID:23138596

Abstract

PURPOSE

The present study was conducted to elucidate the potential of selenium supplementation, if any, in affording chemoprevention by modulating the altered cancer markers and ultrastructural changes in dimethyl hydrazine (DMH)-induced colorectal carcinogenesis in rats.

METHODS

The rats were segregated into four groups, viz., normal control, DMH treated, selenium treated, and DMH + selenium treated. Initiation and induction of colon carcinogenesis was achieved through weekly subcutaneous injections of DMH (30 mg/kg body weight) for both 10 and 20 weeks. Selenium was supplemented to rats at a dose level of 1 ppm in drinking water ad libitum for two different time durations of 10 and 20 weeks.

RESULTS

The study observed a significant increase in the number of aberrant crypt foci (ACF) in colons of DMH-treated rats at both time intervals which were decreased significantly upon selenium supplementation. Also, a significant increase was seen in the enzyme activity of alkaline phosphatase (ALP), which, however, was moderated upon selenium administration to DMH-treated rats. Changes in the ultrastructural architecture of colonic cells were apparent following both the treatment schedules of DMH; however, the changes were prominent following 20 weeks of DMH treatment. The most obvious changes were seen in the form of altered nuclear shape and disruption of cellular integrity, which, upon selenium supplementation, were appreciably improved.

CONCLUSION

In conclusion, the study shows the chemopreventive abilities of selenium against DMH-induced colorectal carcinogenesis in rats.

摘要

目的

本研究旨在阐明补充硒（若有）通过调节二甲基肼（DMH）诱导的大鼠结直肠癌发生过程中改变的癌症标志物和超微结构变化来提供化学预防的潜力。

方法

将大鼠分为四组，即正常对照组、DMH处理组、硒处理组和DMH + 硒处理组。通过每周皮下注射DMH（30 mg/kg体重）持续10周和20周来启动和诱导结肠癌发生。在10周和20周这两个不同时间段内，以1 ppm的剂量水平在大鼠饮用水中随意补充硒。

结果

研究观察到，在两个时间间隔内，DMH处理的大鼠结肠中异常隐窝灶（ACF）的数量显著增加，而补充硒后显著减少。碱性磷酸酶（ALP）的酶活性也显著增加，然而，在给DMH处理的大鼠施用硒后，这种增加得到了缓解。在DMH的两种处理方案后，结肠细胞的超微结构变化都很明显；然而，在DMH处理20周后变化更为突出。最明显的变化表现为核形状改变和细胞完整性破坏，补充硒后这些变化得到明显改善。