一种用于测定血浆中2-甲基磺酰基吡啶的简单高效液相色谱法。

A simple high-performance liquid chromatographic method for the measurement of 2-methylsulfonylpyridine in plasma.

作者信息

Jasheway D W, Park Y H, Mayer P R

机构信息

Alcon Laboratories, Pharmacokinetics/Drug Metabolism, Ft. Worth, TX 76134.

出版信息

J Anal Toxicol. 1990 Jan-Feb;14(1):54-7. doi: 10.1093/jat/14.1.54.

DOI:10.1093/jat/14.1.54

PMID:2314064

Abstract

A simple and sensitive high-performance liquid chromatographic (HPLC) method for the analysis of 2-methylsulfonylpyridine (2-MSP) in plasma has been developed. Up to 1 mL of plasma containing 2-MSP and an internal standard was extracted with 3 mL of methylene chloride, usually twice, evaporated to dryness, resuspended in mobile phase, and chromatographed on two 15-cm C8 reversed-phase LC columns in series. The mobile phase was 5% acetonitrile in water with a flow rate of 1 mL/min and ultraviolet detection was at 260 nm. Extraction of plasma produced no interfering endogenous components and the recovery of 2-MSP was 60-70%. The intraday and interday statistics of 2-MSP standard curves from 10 to 320 ng in plasma demonstrate that the assay is sensitive and precise using either human or monkey plasma and any plasma volume up to 1 mL.

摘要

已开发出一种简单且灵敏的高效液相色谱（HPLC）方法，用于分析血浆中的2-甲基磺酰基吡啶（2-MSP）。将含有2-MSP和内标的至多1 mL血浆用3 mL二氯甲烷萃取，通常萃取两次，蒸发至干，再用流动相复溶，然后在两根串联的15 cm C8反相液相色谱柱上进行色谱分析。流动相为5%乙腈水溶液，流速为1 mL/min，紫外检测波长为260 nm。血浆萃取未产生干扰内源性成分，2-MSP的回收率为60-70%。血浆中2-MSP标准曲线在10至320 ng范围内的日内和日间统计数据表明，该测定法使用人或猴血浆以及至多1 mL的任何血浆体积时均灵敏且精确。