Departament de Medicina Preventiva, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain.
Food Chem. 2011 Jul 15;127(2):822-6. doi: 10.1016/j.foodchem.2011.01.016. Epub 2011 Jan 9.
A sensitive and selective liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-ESI-MS-MS) method was developed for the routine analysis of aflatoxins (AFB(1), AFB(2), AFG(1) and AFG(2)) and ochratoxin A (OTA) in tiger nuts and tiger-nut beverage (horchata). A matrix solid phase dispersion was adapted to eliminate lipidic interferences. The solid support was C(18), while the elution solvent was acetonitrile. Mean recoveries obtained at two fortification levels were 72-83% and 71-81% for horchata and tiger nut respectively with relative standard deviations (RSDs) <13% and 15% respectively. The LC-MS-MS method allowed quantification and identification at low levels in two matrices. The method was applied for the routine analysis of tiger-nuts and horchata samples collected from different supermarkets of Valencia (Spain) during one year (March 2009-March 2010). A total of 238 samples were analysed and 32 samples were found positives for OTA, AFB(1), AFB(2) and AFG(2).
建立了一种灵敏、选择性的液相色谱-电喷雾串联三重四极杆质谱法（LC-ESI-MS-MS），用于常规分析油莎豆及其饮料（虎坚果奶）中的黄曲霉毒素（AFB1、AFB2、AFG1 和 AFG2）和赭曲霉毒素 A（OTA）。采用基质固相分散法消除脂质干扰。固体支撑物为 C18，洗脱溶剂为乙腈。在两个加标水平下，虎坚果奶和油莎豆的平均回收率分别为 72-83%和 71-81%，相对标准偏差（RSD）分别小于 13%和 15%。LC-MS-MS 方法允许在两种基质中进行低水平的定量和鉴定。该方法应用于对 2009 年 3 月至 2010 年 3 月期间从西班牙瓦伦西亚不同超市采集的油莎豆和虎坚果奶样本进行常规分析。共分析了 238 个样本，其中 32 个样本 OTA、AFB1、AFB2 和 AFG2 检测为阳性。
