Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.
Theriogenology. 2013 Jan 15;79(2):306-11.e1. doi: 10.1016/j.theriogenology.2012.08.021. Epub 2012 Nov 8.
The objective was to examine the effects of valproic acid (VPA), a histone deacetylase inhibitor, on in vitro and in vivo development of Wuzhishan miniature pig somatic cell nuclear transfer (SCNT) embryos. Experiment 1 compared in vitro developmental competence of nuclear transfer embryos treated with various concentrations of VPA for 24 h. Embryos treated with 2 mM VPA for 24 h had a greater rate of blastocyst formation compared with control or embryos treated with 4 or 8 mM VPA (21.5% vs. 10.5%, 12.6%, and 17.2%, P < 0.05). Experiment 2 examined the in vitro developmental competence of nuclear transfer embryos treated with 2 mM VPA for various intervals after chemical activation. Embryos treated for 24 h had higher rates of blastocyst formation than the control or those treated for 4 or 48 h (20.7% vs. 9.2%, 12.1%, and 9.1%, P < 0.05). In Experiment 3, an average of 207 (range, 192-216) nuclear transfer embryos from the VPA-treated group were transferred to surrogate mothers, resulting in three pregnancies. Two of the surrogates delivered a total of 11 live piglets. However, for unknown reasons, nine of 11 piglets in the VPA-treated group died within 1 to 5 d after birth. Untreated control embryos (average, 205; range, 179-225) transferred to four surrogate mothers resulted in three pregnancies, two of which delivered a total of 12 live offspring, although four of 12 piglets in the VPA-untreated group died (cause unknown) within 1 to 3 d, whereas eight of the 12 piglets in the VPA-untreated group survived more than 3 or 4 mo. The average birth weight of the two litters from the VPA-treated group tended (P < 0.05) to be lower than that from the control groups (551.6 g vs. 675.2 g). In conclusion, VPA treatment increased the blastocyst formation rate of SCNT porcine embryos; both VPA-treated and the untreated clones developed to term, but offspring from VPA-treated embryos had a lower survival to adulthood than those from control embryos (18.2% vs. 67.0%; P < 0.05).
目的是研究组蛋白去乙酰化酶抑制剂丙戊酸（VPA）对五指山小型猪体细胞核移植（SCNT）胚胎体外和体内发育的影响。实验 1 比较了用不同浓度 VPA 处理 24 小时的核移植胚胎的体外发育能力。与对照组或用 4 或 8mM VPA 处理的胚胎相比，用 2mM VPA 处理 24 小时的胚胎囊胚形成率更高（21.5%比 10.5%、12.6%和 17.2%，P<0.05）。实验 2 研究了用 2mM VPA 处理化学激活后的核移植胚胎不同时间间隔的体外发育能力。与对照组或处理 4 或 48 小时的胚胎相比，处理 24 小时的胚胎囊胚形成率更高（20.7%比 9.2%、12.1%和 9.1%，P<0.05）。在实验 3 中，从 VPA 处理组中取出 207 个（范围为 192-216 个）核移植胚胎转移到代孕母亲体内，导致 3 次怀孕。其中 2 个代孕母亲共产下 11 头活产仔猪。然而，由于未知原因，VPA 处理组的 11 头仔猪中有 9 头在出生后 1-5 天内死亡。未经处理的对照组胚胎（平均 205 个；范围 179-225 个）转移到 4 个代孕母亲体内，导致 3 次怀孕，其中 2 次共产下 12 头活产仔猪，而未经 VPA 处理组的 12 头仔猪中有 4 头（原因不明）在 1-3 天内死亡，而未经 VPA 处理组的 12 头仔猪中有 8 头存活超过 3 或 4 个月。VPA 处理组的两窝仔猪的平均出生体重（P<0.05）低于对照组（551.6g 比 675.2g）。结论：VPA 处理提高了 SCNT 猪胚胎的囊胚形成率；VPA 处理和未处理的克隆都发育到足月，但 VPA 处理组的后代存活到成年的比例低于对照组（18.2%比 67.0%；P<0.05）。
