Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
J Inorg Biochem. 2013 Jan;118:187-90. doi: 10.1016/j.jinorgbio.2012.10.004. Epub 2012 Oct 12.
Nitroxyl (HNO) is a small molecule with various pharmacological effects, including cardioprotective action. It is thought to serve as a modulator of various biochemical pathways. But, it is difficult to apply HNO directly for biological experiments or therapeutic treatment because it is highly reactive, readily dimerizing or reacting with biological targets under ambient conditions. Therefore, HNO donor molecules that release HNO under physiological conditions, especially those that allow precisely controllable release, would be useful to study the activities of HNO at the cellular level. This short review focuses on recently developed photocontrollable HNO-releasing compounds, which are expected to be suitable for achieving site-specific and temporally controlled HNO release in biomedical investigations. An illustrative application for the study of HNO-mediated upregulation of calcitonin gene-related peptide (CGRP) in A549 cells is described.
硝普氢（HNO）是一种具有多种药理作用的小分子，包括心脏保护作用。它被认为是各种生化途径的调节剂。但是，由于 HNO 高度反应性，在环境条件下容易二聚化或与生物靶标反应，因此直接将 HNO 应用于生物实验或治疗处理是困难的。因此，在生理条件下释放 HNO 的 HNO 供体分子，特别是那些允许精确可控释放的 HNO 供体分子，将有助于研究 HNO 在细胞水平上的活性。本综述重点介绍了最近开发的光控 HNO 释放化合物，预计它们可用于在生物医学研究中实现特定部位和时间控制的 HNO 释放。描述了用于研究 HNO 介导的 A549 细胞中降钙素基因相关肽（CGRP）上调的应用实例。
