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从患病的金鲳鱼(Trachinotus ovatus Linnaeus)中分离美人鱼发光杆菌亚种的研究和抗菌剂敏感性。

Studies on the isolation of Photobacterium damselae subsp. piscicida from diseased golden pompano (Trachinotus ovatus Linnaeus) and antibacterial agents sensitivity.

机构信息

South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangdong Province, Guangzhou 510300, China; Key Laboratory of Fishery Ecology and Environment, Guangdong Province, Guangzhou 510300, China; Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangdong Province, Guangzhou 510300, China.

South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangdong Province, Guangzhou 510300, China.

出版信息

Vet Microbiol. 2013 Mar 23;162(2-4):957-963. doi: 10.1016/j.vetmic.2012.09.020. Epub 2012 Sep 27.

Abstract

An epizootic occurred among cultured golden pompano (Trachinotus ovatus); it involved mass mortality on fish farms in Linshui in Hainan province, China, in 2008. Diseased fish exhibited no obvious clinical signs, but pathological studies showed that nodules were scattered over the spleen and kidney. To investigate the nature of the pathogen, we studied the surviving fish, and a Gram-negative bacterium (designated strain TOS1) was isolated from the spleens of golden pompano. Pathogenicity assays revealed that TOS1 was virulent for golden pompano when they were challenged by intraperitoneal injection, and the lethal dose (LD(50)) was 1.1 × 10(6)colony forming units (CFU)g(-1). 16S rDNA gene sequence of TOS1 demonstrated high similarity (99%) to that of Photobacterium damselae subsp. piscicida. Phylogenetic analysis also showed a clear association of strain TOS1 with P. damselae subsp. piscicida, and this agreed with the results of morphological, physiological and biochemical identification. The results also showed that TOS1 was sensitive to norfloxacin, gentamicin, sulfamethoxazole/trimethoprim, neomycin, streptomycin and tetracycline, and was very sensitive to Psidium guajava and Atractylodes lancea (minimum bactericidal concentration, MBC=10(-6)g/mL). This paper describes a systematic study of P. damselae subsp. piscicida isolated from diseased golden pompano in China, including its sensitivity to different antibiotics and Chinese herbal extracts, which will contribute to the diagnosis and prevention of the associated disease.

摘要

2008 年,中国海南省临水产的养殖金目鲈(Trachinotus ovatus)发生了一次流行病,大量鱼类死亡。患病鱼没有明显的临床症状,但病理学研究表明,脾脏和肾脏有散在的结节。为了研究病原体的性质,我们对存活的鱼进行了研究,从金目鲈的脾脏中分离出了一株革兰氏阴性菌(命名为 TOS1 株)。致病性试验表明,TOS1 株对金目鲈具有致病性,通过腹腔注射攻毒,其半数致死剂量(LD50)为 1.1×106CFUg-1。TOS1 株的 16S rDNA 基因序列与迟缓爱德华氏菌(Photobacterium damselae subsp. piscicida)高度相似(99%)。系统发育分析也表明,TOS1 株与迟缓爱德华氏菌(Photobacterium damselae subsp. piscicida)密切相关,这与形态学、生理学和生物化学鉴定的结果一致。结果还表明,TOS1 株对诺氟沙星、庆大霉素、磺胺甲噁唑/甲氧苄啶、新霉素、链霉素和四环素敏感,对番石榴和白术(最小杀菌浓度,MBC=10-6g/mL)非常敏感。本文描述了对中国患病金目鲈中分离出的迟缓爱德华氏菌(Photobacterium damselae subsp. piscicida)的系统研究,包括其对不同抗生素和中草药提取物的敏感性,这将有助于相关疾病的诊断和预防。

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