Núñez-Díaz José Alberto, Fumanal Milena, do Vale Ana, Fernández-Díaz Catalina, Moriñigo Miguel Ángel, Balebona María Carmen
Departamento de Microbiología, Universidad de Málaga, Andalucia Tech, Campus de Teatinos s/n, 29071 Málaga, Spain.
Fish Immunology and Vaccinology Group, IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal.
Microorganisms. 2018 Jul 12;6(3):67. doi: 10.3390/microorganisms6030067.
subsp. () is responsible for disease outbreaks in marine aquaculture worldwide. , a valuable fish species for aquaculture in the south of Europe, is frequently affected by this pathogen. It is well established that bacteria respond to environmental signals and, in the case of pathogens, this ability may determine the outcome of their interaction with the host. Determination of gene expression under in vivo conditions constitutes a valuable tool in the assessment of microbial pathogenesis. Considering that different hosts may represent different environments for the pathogen, expression of virulence and in vivo induced antigen (IVIAT) genes during infection has been determined in the present work. Increased transcription of genes encoding proteins involved in iron acquisition (Irp1, Irp2, HutB and HutD), oxidative stress defence (AhpC and Sod), adhesion (PDP_0080), toxins (AIP56) and metabolism (Impdh, Shmt and AlaRS) were detected in infecting head kidney or liver. The highest increases corresponded to genes involved in survival under iron limiting conditions and oxidative stress, indicating their essential role during infection of sole. Results obtained give insight into virulence strategies and contribute to the identification of promising targets for the control of photobacteriosis.
亚种（）是全球海水养殖中疾病爆发的原因。作为欧洲南部水产养殖中有价值的鱼类品种，经常受到这种病原体的影响。众所周知，细菌会对环境信号做出反应，对于病原体而言，这种能力可能决定它们与宿主相互作用的结果。在体内条件下测定基因表达是评估微生物致病性的一种有价值的工具。考虑到不同宿主可能对病原体代表不同的环境，在本研究中已确定了在感染过程中毒力和体内诱导抗原（IVIAT）基因的表达。在感染大菱鲆的头肾或肝脏中检测到参与铁获取（Irp1、Irp2、HutB和HutD）、氧化应激防御（AhpC和Sod）、粘附（PDP_0080）、毒素（AIP56）和代谢（Impdh、Shmt和AlaRS）的蛋白质编码基因的转录增加。最高的增加对应于参与铁限制条件下生存和氧化应激的基因，表明它们在大菱鲆感染过程中的重要作用。获得的结果有助于深入了解大菱鲆的毒力策略，并有助于确定控制发光细菌病的有前景的靶点。
