Cai Ren-mei, Weng Zhan-ping, Wang Yun-ying, Li Yan-ting, Ji Xiang-hong
Department of Obstetrics, Qingdao Municipal Hospital, Qingdao 266071, China.
Zhonghua Fu Chan Ke Za Zhi. 2012 Jul;47(7):510-3.
To investigate the relationship of S100B protein expression and the pathogenesis of early-onset and late-onset preeclampsia.
Sixty patients with preeclampsia who received caesarean section at Qingdao Municipal Hospital from October 2010 to September 2011 were enrolled in this study. Thirty cases were early-onset preeclampsia (referred as early-onset preeclampsia group, < 34 weeks), and the other 30 cases were late-onset preeclampsia (referred as late-onset preeclampsia group, ≥ 34 weeks). Thirty women who received caesarean section because of pelvic structural deformities, breech presentation, macrosomia and social factors were included as the control group. The expression of S100B mRNA in the placenta was detected by reverse transcription (RT)-PCR. The expression of S100B protein in the placenta was detected by immunohistochemistry.
(1) S100B mRNA was expressed in the trophoblasts of preeclampsia and control groups. The expression of S100B mRNA in early-onset preeclampsia group (0.73 ± 0.11) was significantly higher than the control group (0.58 ± 0.08) and late-onset preeclampsia group (0.64 ± 0.10, P < 0.05). There was no significant difference between late-onset preeclampsia group and the control group (P > 0.05). (2) S100B protein was expressed in the plasma membrane and cytoplasm of the trophoblasts, correlated positively with the brownish yellow and brown particles inside the cells. It was expressed in all the three groups. Immunohistochemistry revealed that the expression of S100B protein in the placenta of early-onset preeclampsia group was 100% (30/30), significantly higher than those of late-onset preeclampsia group and the control group, in which the positive rate were 70% (21/30) and 63% (19/30) respectively (P < 0.05). There was no difference between late-onset preeclampsia group and the control group (P > 0.05).
Early-onset and late-onset preeclampsia may have different etiology and pathogenesis. S100B may be a factor in the pathogenesis of early-onset preeclampsia.
探讨S100B蛋白表达与早发型和晚发型子痫前期发病机制的关系。
选取2010年10月至2011年9月在青岛市立医院行剖宫产的60例子痫前期患者。其中30例为早发型子痫前期（早发型子痫前期组，孕周<34周），另外30例为晚发型子痫前期（晚发型子痫前期组，孕周≥34周）。选取30例因骨盆结构畸形、臀位、巨大儿及社会因素行剖宫产的产妇作为对照组。采用逆转录（RT）-PCR法检测胎盘组织中S100B mRNA的表达。采用免疫组织化学法检测胎盘组织中S100B蛋白的表达。
（1）子痫前期组和对照组的滋养细胞均有S100B mRNA表达。早发型子痫前期组S100B mRNA表达水平为（0.73±0.11），显著高于对照组（0.58±0.08）和晚发型子痫前期组（0.64±0.10，P<0.05）。晚发型子痫前期组与对照组比较差异无统计学意义（P>0.05）。（2）S100B蛋白在滋养细胞质膜和胞质中表达，与细胞内棕黄色和棕色颗粒呈正相关。三组均有表达。免疫组织化学显示，早发型子痫前期组胎盘组织中S100B蛋白表达阳性率为100%（30/30），显著高于晚发型子痫前期组和对照组，晚发型子痫前期组和对照组阳性率分别为70%（21/30）和63%（19/30）（P<0.05）。晚发型子痫前期组与对照组比较差异无统计学意义（P>0.05）。
早发型和晚发型子痫前期可能具有不同的病因和发病机制。S100B可能是早发型子痫前期发病机制中的一个因素。
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