Sang Hongai, Ma Yuyan, Zhu Xiaodan, Wang Linlin, He Mengya
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China.
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China; Email:
Zhonghua Fu Chan Ke Za Zhi. 2015 Jun;50(6):441-5.
To investigate the expression of growth arrest-specific protein 6 (Gas6) in the placenta and decidua tissues and its relationship with the pathogenesis of preeclampsia.
All the patients were recruited in Qilu Hospital of Shangdong University from October 2013 to June 2014. Among them, thirty-two women with early-onset severe preeclampsia who received cesarean section were assigned to the preeclampsia group, and thirty healthy pregnant women who received cesarean section were defined as the control group. Blood glucose, blood lipids, platelet count, D-dimer levels and other clinical indicators of the two groups were detected. Immunohistochemistry of SP was conducted to identify the localization of Gas6 protein in the placenta and decidua tissues. And reverse transcription (RT)-PCR was performed for quantitative analysis of Gas6 RNA expression in placentas. The correlations between placental Gas6 mRNA levels with clinical indicators were analyzed.
(1) The gestational age at delivery, blood pressure, serum albumin, platelet count and birth weight of fetuses showed statistically significant differences between the two groups (P < 0.05). (2) The Gas6 protein expressed in the cytoplasm and nucleus of the syncytiotrophoblasts and decidual cells in the placenta and decidual tissues of the two groups. (3) The Gas6 mRNA expression elevated significantly in the placenta of preeclampsia group (0.60 ± 0.38) when compared to that of the control group (0.34 ± 0.22; P < 0.05). (4) The expression of Gas6 mRNA was positively related with body mass index, diastolic blood pressure, systolic blood pressure, free fatty acids and creatinine (P < 0.05), while it was negatively associated with serum albumin (P < 0.05).
The high expression of Gas6 in the placenta and decidua tissues may be related to the pathogenesis of severe preeclampsia.
探讨生长抑制特异性蛋白6（Gas6）在胎盘和蜕膜组织中的表达及其与子痫前期发病机制的关系。
选取2013年10月至2014年6月在山东大学齐鲁医院就诊的患者。其中，32例早发型重度子痫前期患者行剖宫产术，归入子痫前期组；30例健康孕妇行剖宫产术，作为对照组。检测两组患者的血糖、血脂、血小板计数、D - 二聚体水平等临床指标。采用免疫组织化学SP法鉴定Gas6蛋白在胎盘和蜕膜组织中的定位。采用逆转录（RT）-PCR法对胎盘组织中Gas6 RNA表达进行定量分析。分析胎盘Gas6 mRNA水平与临床指标之间的相关性。
（1）两组患者的分娩孕周、血压、血清白蛋白、血小板计数及胎儿出生体重比较，差异有统计学意义（P < 0.05）。（2）两组胎盘和蜕膜组织的合体滋养层细胞和蜕膜细胞的细胞质及细胞核中均有Gas6蛋白表达。（3）子痫前期组胎盘组织中Gas6 mRNA表达水平（0.60±0.38）显著高于对照组（0.34±0.22；P < 0.
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