Valor L, Sarmiento E, Navarro J, Gallego A, Fernandez-Yañez J, Fernandez-Cruz E, Carbone J
Clinical Immunology Department, Gregorio Marañon University Hospital, Madrid, Spain.
Transplant Proc. 2012 Nov;44(9):2649-52. doi: 10.1016/j.transproceed.2012.09.054.
The analysis of proliferative responses using 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE) in flow cytometry is widely used to assess lymphocyte function. The aim of this study was to evaluate nonspecific and specific lymphoproliferative responses using CFSE in heart recipients before and after transplantation and their association with the development of infection. We used four-color flow cytometry to measure the response of peripheral CD3+, CD4+, and CD8+ T cells to phytohemagglutinin mitogen (PHA), tetanus toxoid, hepatitis B, and influenza vaccines using a CFSE proliferation assay in 12 heart recipients and 8 healthy control subjects. Recipients were prospectively evaluated. Immunological studies were performed before and at 3 months after transplantation. A 12-month clinical follow-up examination sought to detect the prevalence of severe infectious complications. Heart recipients (infected [n = 7] and uninfected [n = 5]) disclosed significantly lower percentages of proliferative responses than healthy controls against PHA at both study points. Baseline CD3+, CD4+, and CD8+, antitetanus proliferative responses were significantly lower in infected heart recipients than controls. Patients who developed infections displayed significantly lower percentages of CD3+CFSE and CD8+CFSE cells to PHA mitogen at 3 months after transplantation versus those without infections. In conclusion, nonspecific T-cell reactivity to PHA was lower in heart recipients with posttransplantation infections.
在流式细胞术中使用5,6 - 羧基荧光素二乙酸琥珀酰亚胺酯（CFSE）分析增殖反应被广泛用于评估淋巴细胞功能。本研究的目的是评估心脏移植受者在移植前后使用CFSE的非特异性和特异性淋巴细胞增殖反应及其与感染发生的关联。我们使用四色流式细胞术，通过CFSE增殖试验，在12名心脏移植受者和8名健康对照受试者中测量外周血CD3⁺、CD4⁺和CD8⁺ T细胞对植物血凝素丝裂原（PHA）、破伤风类毒素、乙肝疫苗和流感疫苗的反应。对受者进行前瞻性评估。在移植前和移植后3个月进行免疫学研究。为期12个月的临床随访检查旨在检测严重感染并发症的发生率。在两个研究时间点，心脏移植受者（感染组[n = 7]和未感染组[n = 5]）针对PHA的增殖反应百分比均显著低于健康对照组。感染的心脏移植受者的基线CD3⁺、CD4⁺和CD8⁺、抗破伤风增殖反应显著低于对照组。与未发生感染的患者相比，发生感染的患者在移植后3个月时针对PHA丝裂原的CD3⁺CFSE和CD8⁺CFSE细胞百分比显著降低。总之，移植后发生感染的心脏移植受者对PHA的非特异性T细胞反应性较低。
