开发一种夹心酶联免疫吸附测定法以检测和测量犬血清铁蛋白水平。

Development of a sandwich enzyme-linked immunosorbent assay to detect and measure serum levels of canine ferritin.

作者信息

Chikazawa Seishiro, Hori Yasutomo, Hoshi Fumio, Kanai Kazutaka, Ito Naoyuki, Sato Jun, Orino Koichi, Watanabe Kiyotaka, Higuchi Sei-ichi

机构信息

Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23-35-1 Higashi, Towada, Aomori, Japan.

出版信息

J Vet Med Sci. 2013 May 2;75(4):515-7. doi: 10.1292/jvms.12-0324. Epub 2012 Nov 13.

DOI:10.1292/jvms.12-0324

PMID:23149482

Abstract

We established a homologous sandwich enzyme-linked immunosorbent assay (ELISA) to measure serum levels of canine ferritin. Our assay uses a rabbit anti-canine heart ferritin polyclonal antibody, and canine heart ferritin as a standard. Serum ferritin concentration in healthy dogs (n=163) was 789 ± 284 ng/ml (mean ± standard deviation), a value higher than reported previously. Confidence levels relating to repeatability, dilution and recovery for this method were high. Therefore, we believe our developed sandwich ELISA will be effective in evaluating serum levels of canine ferritin.

摘要

我们建立了一种同源夹心酶联免疫吸附测定法（ELISA）来测量犬血清铁蛋白水平。我们的测定法使用兔抗犬心脏铁蛋白多克隆抗体，并以犬心脏铁蛋白作为标准品。健康犬（n = 163）的血清铁蛋白浓度为789±284 ng/ml（平均值±标准差），该值高于先前报道的值。该方法在重复性、稀释和回收率方面的置信水平很高。因此，我们相信我们开发的夹心ELISA将有效地评估犬血清铁蛋白水平。