Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Nat Commun. 2012;3:1206. doi: 10.1038/ncomms2209.
Alterations in the methylation of promoters of cancer-related genes are promising biomarkers for the early detection of disease. Compared with single methylation alteration, assessing combined methylation alterations can provide higher association with specific cancer. Here we use cationic conjugated polymer-based fluorescence resonance energy transfer to quantitatively analyse DNA methylation levels of seven colon cancer-related genes in a Chinese population. Through a stepwise discriminant analysis and cumulative detection of methylation alterations, we acquire high accuracy and sensitivity for colon cancer detection (86.3 and 86.7%) and for differential diagnosis (97.5 and 94%). Moreover, we identify a correlation between the CpG island methylator phenotype and clinically important parameters in patients with colon cancer. The cumulative analysis of promoter methylation alterations by the cationic conjugated polymer-based fluorescence resonance energy transfer may be useful for the screening and differential diagnosis of patients with colon cancer, and for performing clinical correlation analyses.
癌症相关基因启动子甲基化的改变是疾病早期检测有前途的生物标志物。与单个甲基化改变相比，评估联合甲基化改变可以提供与特定癌症更高的关联。在这里，我们使用阳离子共轭聚合物基荧光共振能量转移来定量分析中国人群中七个结肠癌相关基因的 DNA 甲基化水平。通过逐步判别分析和甲基化改变的累积检测，我们获得了结肠癌检测的高准确性和高灵敏度（86.3%和 86.7%）和鉴别诊断（97.5%和 94%）。此外，我们发现了结肠癌患者 CpG 岛甲基化表型与临床重要参数之间的相关性。阳离子共轭聚合物基荧光共振能量转移的启动子甲基化改变累积分析可能有助于结肠癌患者的筛查和鉴别诊断，并进行临床相关性分析。
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